I7633

IMDM

With ʟ-glutamine and HEPES, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(e): IMDM
• UNSPSC Code: 12352207
• NACRES: NA.75
• MDL number: MFCD00217469

Eigenschaften

• Produktname: Modifiziertes Dulbecco-Medium nach Iscove, With L-glutamine and 25 mM HEPES, without sodium bicarbonate, powder, suitable for cell culture
• form: powder
• technique(s): cell culture | hybridoma: suitable, cell culture | mammalian: suitable
• components: phenol red: yes; glucose: yes; L-glutamine: yes
• shipped in: ambient
• storage temp.: 2-8°C

Beschreibung

General description

Iscove′s Modified Dulbecco′s medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids, and vitamins, sodium pyruvate, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and potassium nitrate instead of ferric nitrate. Iscove′s medium supports murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Application

Iscove′s Modified Dulbecco′s Medium has been used to culture KG1a cells (human leukemic cell line)[1][2] and Chinese hamster ovary (CHO) DG44 cell line.[3]

Reported to support murine B lymphocytes, hematopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Preparation Note

Formulated to contain 17.7 grams of powder per liter of medium.

Supplement with 3.024 g/L sodium bicarbonate.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable