The specific buffer formulation used to prepare this product is considered proprietary. The buffer includes primarily Tris base, with sodium chloride and magnesium chloride (hexahydrate form), as well as a minimal amount of citric acid. Please see the link below to review additional information available in the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/420/993/i1643pis.pdf
Fortfahren mit
I1643
Pyrophosphatase, anorganisch aus Backhefe (S. cerevisiae)
powder, ≥500 units/mg protein (E1%/280)
Synonym(e):
Anorganische Pyrophosphatase aus E. coli, Pyrophosphate phosphohydrolase
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 100 units | Warenkorb auf Verfügbarkeit prüfen | € 169,00 | |
| 1000 units | Warenkorb auf Verfügbarkeit prüfen | € 533,00 | |
| 500 units | Warenkorb auf Verfügbarkeit prüfen | € 668,00 |
Über diesen Artikel
CAS-Nummer:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-784-7
MDL number:
Specific activity:
≥500 units/mg protein (E1%/280)
€ 169,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenform
powder
Quality Segment
specific activity
≥500 units/mg protein (E1%/280)
mol wt
71 kDa
storage temp.
−20°C
General description
pI: 4.75
Application
Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PPi + H2O → 2Pi.
It plays an important role in protein, RNA, and DNA synthesis.
It plays an important role in protein, RNA, and DNA synthesis.
Pyrophosphatase has been used:
Pyrophosphatase has been used:
as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.
as a component of PCR mixture
as a component of the reaction buffer used in aminoacylation assay to quantify incorporation of [3H]L-methionine into tRNA.
as a component of PCR mixture
Physical form
Lyophilized powder containing 80-90% buffer salts
Other Notes
One unit will liberate 1.0 μmole of inorganic orthophosphate per min at pH 7.2 at 25 °C.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| specific activity ≥500 units/mg protein (E1%/280) | specific activity ≥500 units/mg protein | specific activity ≥400 units/mg protein (biuret) | specific activity ≥50 units/mg protein |
| form powder | form ammonium sulfate suspension | form ammonium sulfate suspension | form lyophilized powder |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| mol wt 71 kDa | mol wt - | mol wt tetramer 119,500 Da±600 | mol wt 93 kDa |
| Quality Level 300 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Verwandter Inhalt
Product Information Sheet
Global Trade Item Number
| SKU | GTIN |
|---|---|
| I1643-100UN | 04061833847916 |
| I1643-500UN | 04061833847930 |
| I1643-1KU | 04061833847923 |
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We would like to make a "blank" without enzyme for the powder resuspended at 2 mg/mL in water. Could you please share the concentration of Tris, citric acid and magnesium chloride?
1 answer-
Helpful?
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I want to know what the endotoxin value is in this product(Pyrophosphatase, Sigma-Aldrich, I1643-1KU, LOT-SLCL2396).
1 answer-
This product is NOT tested for endotoxin. Please see the Certificate of Analysis for testing that is performed.
Helpful?
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