H7041

IL-2 human

≥98% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• Synonym(e): Interleukin-2, human, T-Zell-Wachstumsfaktor
• UNSPSC Code: 12352202
• NACRES: NA.77
• MDL number: MFCD00165721
• Biological source: human
• Recombinant: expressed in HEK 293 cells
• Form: lyophilized powder
• Mol wt: monomer 15 kDa (glycosylated)

Eigenschaften

• Produktname: Interleukin-2 human, IL-2, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin tested
• biological source: human
• Quality Segment: 100
• recombinant: expressed in HEK 293 cells
• form: lyophilized powder
• potency: ≤5.0 ng/mL ED₅₀
• quality: endotoxin tested
• mol wt: monomer 15 kDa (glycosylated)
• packaging: pkg of 5X10 μg, pkg of 10 μg, pkg of 100 μg
• technique(s): cell culture | mammalian: suitable
• UniProt accession no.: P60568
• storage temp.: −20°C
• Gene Information: human ... IL2(3558)

Beschreibung

General description

Interleukin-2 (IL-2) is an α-helical cytokine belonging to T-helper cell type 1. It has a molecular weight of 15kDa. The IL2 gene is mapped to human chromosome 4q27. IL-2 is originally called as T cell growth factor.

Application

Interleukin-2 human has been used to culture peripheral blood mononuclear cells (PBMC). It has been used to activate effector cell (PBMC) and to study antibody-dependent cell-mediated cytotoxicity.

Biochem/physiol Actions

Interleukin-2 (IL-2) ist ein immunmodulierender Faktor, der von spezifischen Untergruppen der T-Lymphozyten produziert wird. Dieses Lymphokin fördert das langfristige Wachstum von aktivierten T-Zellen und verwandten Zelltypen. IL-2 spielt eine Rolle bei der Aktivierung und Proliferation von NK-Zellen, induziert die Sekretion von γ-Interferon und B-Zell-Wachstumsfaktor und moduliert die Expression des IL-2-Rezeptors.

Interleukin-2 (IL-2) is essential for proliferation of natural killer (NK) cells, lymphocytes and macrophages. It plays a key role in the immune system functions such as NK cell-stimulated immune responses. IL-2 also mediates the formation and essential functions of regulatory T cells. It is implicated in autoimmune disease. IL-2 is associated with apoptosis and thus, possesses antitumor response. IL-2 mediates CD41⁺ T-cells differentiation into T-helper cell 1 and 2 effector subsets, preventing T-helper 17 differentiation.

Preparation Note

Human Interleukin-2 (IL-2) expressed in human HEK 293 cells is a glycosylated monomer with a molecular mass of 15 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line).

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable