I1645

Interleukin-8 human

≥98% (SDS-PAGE and HPLC), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• Synonym(e): IL-8
• MDL-Nummer: MFCD00131404
• UNSPSC-Code: 12352202
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Qualitätsniveau: 100
• Rekombinant: expressed in E. coli
• Assay: ≥98% (SDS-PAGE and HPLC)
• Form: lyophilized powder
• Mol-Gew.: 8.4 kDa
• Verpackung: pkg of 10 μg
• Methode(n): cell culture | mammalian: suitable
• Verunreinigungen: endotoxin, tested
• UniProt-Hinterlegungsnummer: P10145
• Lagertemp.: −20°C
• Angaben zum Gen: human ... IL8(3576)

Beschreibung

Anwendung

Interleukin-8 (IL-8) human has ben used to:

• measure neutrophil chemotoxis[1][2]
• study the uptake of apoptotic cells by meningothelial cells[3]
• study the effect of IL-8 on expression and secretion of urocortin-1 by human umbilical vein endothelial cells[4]

Biochem./physiol. Wirkung

Interleukin-8 (IL-8) was formerly called monocyte-derived neutrophil chemotactic factor. It belongs to the chemokine α or C-X-C family. Like other members of this family, the mature form of IL-8 has four cysteine residues and the first two cysteine residues are separated by glutamine. IL-8 shows chemotactic activity in vitro for T cells, neutrophils and basophils.[5] Mutation in IL-8 can increase risk to acute pancreatitis.[6]

Interleukin-8 (IL-8), formerly called monocyte-derived neutrophil chemotactic factor, belongs to the chemokine α or C-X-C family. The mature form of IL-8 has 4 cysteine residues, as do the other members of the chemokine family and the first two cysteine residues are separated by glutamine. Mature human IL-8 consists of 72 amino acids with a molecular mass of 8.4 kDa. IL-8 exhibits chemotactic activity in vitro for T cells, basophils, as measured by enzymes including myeloperoxidase, α-mannosidase and β-glucuronidase. IL-8 is an angiogenic factor.

Physikalische Form

Lyophilized from a 0.2 μm filtered solution with no additives.

Hinweis zur Analyse

The biological activity is determined by its ability to chemoattract human peripheral blood neutrophils using a concentration range of 10.0-100.0 ng/ml.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)