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H6909

Hydrocortison -Lösung

50 μM, sterile-filtered, BioXtra, suitable for cell culture

Synonym(e):

17-Hydroxykortikosteron, Cortisol

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Ihnen/SKUVerfügbarkeitPreis
10 mL
Warenkorb auf Verfügbarkeit prüfen
€ 106,00

Über diesen Artikel

CAS-Nummer:
NACRES:
NA.77
PubChem Substance ID:
UNSPSC Code:
12352209
MDL number:
Biological source:
non-animal source
Form:
liquid

€ 106,00


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biological source

non-animal source

Quality Segment

sterility

sterile-filtered

product line

BioXtra

form

liquid

concentration

50 μM

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

5.0- 7.0

shipped in

dry ice

storage temp.

−20°C

SMILES string

C[C@]12CCC(=O)C=C1CC[C@H]3[C@@H]4CC[C@](O)(C(=O)CO)[C@@]4(C)C[C@H](O)[C@H]23

InChI

1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1

InChI key

JYGXADMDTFJGBT-VWUMJDOOSA-N

Application

Hydrocortison wird in epithelialen und endothelialen adhärenten Zellkulturanwendungen eingesetzt. Hydrocortisonlösung wird zur Isolation von epidermalen Stammzellen und zur Zellexpansion eingesetzt.[1] Es wird außerdem im Keratinozyten-Permeabilitätsassay eingesetzt.[2]

Physical form

In Arbeitsaliquoten eingefroren, wiederholtes Einfrieren/Auftauen ist zu vermeiden

Other Notes

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Dieser Artikel
H0135H3160H4001
biological source

non-animal source

biological source

synthetic (organic)

biological source

plant (wild yam)

biological source

synthetic (organic)

product line

BioXtra

product line

BioXtra

product line

-

product line

-

Quality Level

500

Quality Level

200

Quality Level

300

Quality Level

300

form

liquid

form

powder

form

powder

form

powder

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

concentration

50 μM

concentration

-

concentration

-

concentration

-


Lagerklasse

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–9 of 9 Questions  
  1. Regarding product H6909, I would like to know the name of the components in which it is diluted and its final concentration.

    1 answer
    1. Product No. H6909 is a solution of hydrocortisone in normal saline (0.85%).

      Helpful?

  2. Dear Madam/Sir, I hope you’re doing well. I am reaching out to ask about the composition of the cortisol solution at a concentration of 50uM. Could you confirm whether the cortisol is dissolved in water, ethanol, or another solvent? Thank you

    1 answer
    1. This product is 50 um Hydrocortisone solution prepared in saline. Ethanol is used to solubilize during preparation. The final ethanol concentration is approximately 0.2%.

      Helpful?

  3. How long is this product stable in cell culture medium (e.g. DMEM) at 4°C, or just 4°C in general?

    1 answer
    1. The stability of this product after being diluted in medium, has not been evaluated. However, various medium products that are supplemented with Hydrocortisone, are stable for up to 6 weeks when stored refrigerated. As an example, see product C-20011, Keratinocyte Growth Medium, at the link below:
      https://www.sigmaaldrich.com/US/en/product/sigma/c20011

      Helpful?

  4. Is it suitable to further dilute small quantities of Product H6909, Hydrocortisone solution, in phosphate-buffered saline to prepare lower concentration aliquots?

    1 answer
    1. It is not recommended to further dilute H6909, Hydrocortisone solution, for storage purposes as stability/integrity of more dilute forms cannot be guaranteed.

      Helpful?

  5. What is the concentration of Product No. H6909, Hydrocortisone solution?

    1 answer
    1. Product No. H6909 is a 50 μM solution. This may also be written as approximately 0.0181 g/L.

      Helpful?

  6. Should Product No. H6909, Hydrocortisone solution, be protected from light?

    1 answer
    1. Yes. Product No. H6909 is packaged in an amber vial.  If making single use aliquots, we recommend to use amber vials to protect the solution from light.

      Helpful?

  7. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  8. Is there ethanol in Product No. H6909, Hydrocortisone solution?

    1 answer
    1. Hydrocortisone is initially solubilized in ethanol and then diluted in normal saline.  The final concentration of ethanol is 2 ml/L (0.2% v/v).

      Helpful?

  9. Is Product No. H6909, Hydrocortisone solution, isotonic?

    1 answer
    1. Product No. H6909 is a solution of hydrocortisone in normal saline (0.85%).  It is an isotonic solution of hydrocortisone.

      Helpful?

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