H6533

Monoklonaler ANTI-HA-Antikörper

clone HA-7, purified from hybridoma cell culture

• Synonym(e): Anti-HA, Anti-Influenza-Hämagglutinin
• UNSPSC Code: 12352203
• NACRES: NA.56
• MDL number: MFCD00803873
• Conjugate: peroxidase conjugate
• Clone: HA-7, monoclonal
• Application: WB
• Citations: 135

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: peroxidase conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: HA-7, monoclonal
• form: lyophilized powder
• packaging: vial of 0.5 mL
• concentration: 5-11 mg/mL
• technique(s): western blot: 1:4,000 using using HA tagged fusion protein expressed in bacteria, and ECL immunoblotting detection reagent
• storage temp.: 2-8°C

Beschreibung

General description

Influenza A virus, a RNA virus, encodes two surface glycoproteins, hemagglutinin (HA) and neuraminidase (NA). HA stalk domain based epitope region antibody is effective in neutralizing divergent influenza virus strains.

The mouse monoclonal Anti-HA-Peroxidase antibody recognizes native and denatured-reduced forms of the HA-tagged proteins. It also reacts with the N- or C-terminal HA-tagged fusion proteins expressed in E. coli and mammalian cells.

Immunogen

An KLH konjugiertes synthetisches Peptid, das einem Fragment des Hämagglutinins (HA) des menschlichen Grippevirus, genannt HA-Tag, entspricht.

Application

Monoclonal Anti-HA-Peroxidase antibody produced in mouse has been used:

• in immunopreciptation and immunostaining of human member 9 of solute carrier family 38 SLC38A9 transfected in HeLa cells[1]
• in immunoblot detection in transfected A375 cells
• is suitable for immunoblotting of nucleosome assembly protein NAP1L1

Biochem/physiol Actions

Der Antikörper erkennt native sowie durch Denaturierung reduzierte Formen des HA-getaggten Proteins und reagiert mit am N- oder C-Endpunkt HA-getaggten Fusionsproteinen, die in E. coli oder in Säugetierzellen exprimiert werden.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Preparation Note

Reconstitute in .5mL of distilled water.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable