H9037

Monoklonaler ANTI-HA-Antikörper

~1 mg/mL, clone HA-7, purified from hybridoma cell culture

• Synonym(e): Anti-HA, Anti-Influenza-Hämagglutinin
• UNSPSC Code: 12352203
• NACRES: NA.56
• MDL number: MFCD00803873
• Conjugate: TRITC conjugate
• Clone: HA-7, monoclonal
• Application: IF (d)
• Citations: 9

Eigenschaften

• biological source: mouse
• Quality Segment: 200
• conjugate: TRITC conjugate
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: HA-7, monoclonal
• form: buffered aqueous solution
• storage condition: protect from light
• concentration: ~1 mg/mL
• technique(s): direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C

Beschreibung

General description

Monoclonal Anti-HA is derived from the HA-7 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Monoclonal anti-HA recognizes the HA tag sequence on HA tagged fusion proteins by immunocytochemistry. The antibody reacts specifically with N- and C-terminal HA tagged fusion proteins.

Immunogen

An KLH konjugiertes synthetisches Peptid, das einem Fragment des Hämagglutinins (HA) des menschlichen Grippevirus, genannt HA-Tag, entspricht.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Monoclonal Anti-HA-TRITC antibody was used to test if DOT/ICM effector SDHA is necessary for virulence of Legionella pneumophila in Galleria mellonella and A/J mice.

Biochem/physiol Actions

Der Antikörper erkennt native sowie durch Denaturierung reduzierte Formen des HA-getaggten Proteins und reagiert mit am N- oder C-Endpunkt HA-getaggten Fusionsproteinen, die in E. coli oder in Säugetierzellen exprimiert werden.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin 15 mM sodium azide as a preservative.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable