D0672

φX174 DNA Marker Hae III Digest

for DNA electrophoresis

• UNSPSC-Code: 41105335
• NACRES: NA.25

Eigenschaften

• Form: liquid
• Verwendung: 200 uses (0.1 mg size); 40 uses (20 μg size)
• Eignung: suitable for electrophoresis (DNA)
• Lagertemp.: −20°C

Beschreibung

Allgemeine Beschreibung

Sigma′s Phi X174 DNA Marker Hae III Digest contains 11 fragments from 72 − 1,353 bp. Five ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Komponenten

Sigma′s Phi X174 DNA Marker Hae III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Angaben zur Herstellung

φX174RF-I DNA was digested with Hae III to completion, then purified by phenol extraction.

Sonstige Hinweise

For optimal resolution, the recommended agarose gel concentration is 2.0%.

Sicherheitshinweise

• Lagerklassenschlüssel: 10 - Combustible liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)