D9780

Lambda DNA Hind III Digest

for DNA electrophoresis

• Synonym(e): Hind III digested bacteria phage Lambda DNA
• NACRES: NA.25
• UNSPSC Code: 41141827
• Form: liquid
• Storage temp.: −20°C

Eigenschaften

• Produktname: Lambda DNA Hind III Digest, for DNA electrophoresis
• grade: Molecular Biology
• form: liquid
• usage: 100 uses (0.1 mg size); 1000 uses (1 mg size); 20 uses (20 μg size); 500 uses (0.5 mg size)
• suitability: suitable for electrophoresis (DNA)
• shipped in: dry ice
• storage temp.: −20°C
• Quality Level: 200

Beschreibung

Application

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

General description

Sigma′s Lambda DNA Hind III Digest contains 8 fragments from 125 – 23,130 bp. Ten ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

Other Notes

Contains 8 fragments, 125–23,130 bp

For optimal resolution, the recommended agarose gel concentration is 0.75%.

Sigma′s Lambda DNA Hind III Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

Preparation Note

Bacteriophage lambda DNA was digested with EcoR I and Hind III to completion, then purified by phenol extraction.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• flash_point_f: Not applicable
• flash_point_c: Not applicable