C3515

Katalase aus Aspergillus niger

ammonium sulfate suspension, ≥4,000 units/mg protein

• Synonym(e): H₂O₂:H₂O₂ Oxidoreduktase
• CAS-Nummer: 9001-05-2
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54
• EG-Nummer: 1.11.1.6 (BRENDA, IUBMB)
• MDL number: MFCD00081483
• Specific activity: ≥4,000 units/mg protein
• Biological source: Aspergillus niger

Eigenschaften

• SMILES string: O(CC)C(=O)c1ccc(cc1)O
• InChI: 1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
• InChI key: NUVBSKCKDOMJSU-UHFFFAOYSA-N
• biological source: Aspergillus niger
• form: ammonium sulfate suspension
• specific activity: ≥4,000 units/mg protein
• mol wt: tetramer ~250 kDa
• storage condition: (Tightly closed)
• technique(s): FISH: suitable
• UniProt accession no.: A2QH56, A2R2G8
• shipped in: wet ice
• storage temp.: 2-8°C
• Quality Level: 200

Beschreibung

General description

Research area: Cell Signaling

Catalase is an active enzyme[1] present in aerobic organisms.[2] It is a ferric hemoprotein and a tetramer.[3]

Application

Catalase from Aspergillus niger has been used:

• as a positive control during the functional characterization of Clostridium difficile spore coat proteins.[4]
• as a component of the catalase solution to prepare GLOX buffer with enzymes to maintain the embryos of Caenorhabditis elegans before single-molecule fluorescence in situ hybridization (smFISH) studies[5]
• as a component of the imaging buffer for stochastic optical reconstruction microscopy (STORM) imaging of platelet-rich plasma[6]
• as a supplement in Todd Hewitt media plus 0.5 % yeast extract (THY) media for the neutralization of pneumococcal H₂O₂[7]

Biochem/physiol Actions

Catalase catalyzes the decomposition of hydrogen peroxide into water and oxygen. Each subunit of the tetramer contains iron bound to a protoheme IX group. The enzyme also strongly binds NADP, which is in close proximity to the heme group. Isoelectric point is found to be 6.5. Optimum pH for catalytic activity is 7.0. The enzyme activity is inhibited by 3-amino-1-H-1,2,4 triazole, cyanide, azide, hydroxylamine, cyanogen bromide, 2-mercaptoethanol, dithiothreitol, dianisidine, and nitrate. Incubation of catalase with ascorbate or ascorbate/Cu²⁺ results in degradation of the catalase molecule.

Catalase is involved in catalyzing the degradation of hydrogen peroxide (H₂O₂) to water and free oxygen. Catalase is used commercially for decomposing H₂O₂ as it is added as an antimicrobial agent in process streams.[8] It also catalyzes the oxidation of electron donors like ethanol and phenols during lower concentrations of H₂O₂.[9] Catalase shows antioxidant activity by protecting cells from higher levels of reactive oxygen species (ROS). Increased expression of catalase is observed in chronic lymphocytic leukemia, gastric cancer, glioma, and melanoma. Decreased levels of catalase are seen in acute myeloid leukemia, lung, pancreatic, prostate, and skin (non-melanoma) cancers. Hence catalase exhibits a dual role in cancer.[2]

Physical form

Suspension in 3.2 M (NH₄)₂SO₄-Lösung, pH 6.0

Analysis Note

Proteingehalt mittels Biuret-Reaktion bestimmt.

Other Notes

1 U baut 1.0 μmol H₂O₂ pro Minute bei pH 7.0 und 25 °C ab, dabei fällt die H₂O₂-Konzentration von 10.3 zu 9.2 mM. Dies wird durch die Abnahme von A₂₄₀ gemessen.

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Disclaimer

Das Gefrieren der Lösungen ist nicht empfehlenswert.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable