The specific site of biotin attachment on the IgG molecule has not been determined.
Additionally, the biotinylation procedure is proprietary and, therefore, cannot be disclosed.
Fortfahren mit
B9183
Monoklonaler ANTI-HA-Antikörper
clone HA-7, purified from hybridoma cell culture
Synonym(e):
Anti-HA, Anti-Influenza-Hämagglutinin
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
Ansicht ändern
| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 μg | Warenkorb auf Verfügbarkeit prüfen | € 449,00 |
Über diesen Artikel
Conjugate:
biotin conjugate
Clone:
HA-7, monoclonal
Application:
WB CL
Citations:
7
€ 449,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenbiological source
mouse
Quality Segment
conjugate
biotin conjugate
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
HA-7, monoclonal
form
buffered aqueous solution
concentration
~1 mg/mL
technique(s)
western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts.
shipped in
dry ice
storage temp.
2-8°C
General description
The Monoclonal Anti-HA Biotin Conjugate antibody recognizes the HA tag sequence on the HA tagged fusion proteins when expressed at the N or C terminal to the fusion protein. This antibody is derived from the HA-7 hybridoma, which is produced from the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH. Ascites fluid collected from the HA-7 hybridoma is then conjugated to biotin.
Immunogen
An KLH konjugiertes synthetisches Peptid, das einem Fragment des Hämagglutinins (HA) des menschlichen Grippevirus, genannt HA-Tag, entspricht.
Application
Antibody suitable for immunoblotting.
Biochem/physiol Actions
Der Antikörper erkennt native sowie durch Denaturierung reduzierte Formen des HA-getaggten Proteins und reagiert mit am N- oder C-Endpunkt HA-getaggten Fusionsproteinen, die in E. coli oder in Säugetierzellen exprimiert werden.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| antibody form purified from hybridoma cell culture | antibody form purified from hybridoma cell culture | antibody form ascites fluid | antibody form affinity isolated antibody |
| clone HA-7, monoclonal | clone HA-7, monoclonal | clone HA-7, monoclonal | clone polyclonal |
| biological source mouse | biological source mouse | biological source mouse | biological source rabbit |
| conjugate biotin conjugate | conjugate TRITC conjugate | conjugate unconjugated | conjugate unconjugated |
| technique(s) western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts. | technique(s) direct immunofluorescence: 10-15 mg/mL using mammalian cells expressing HA-tagged fusion proteins | technique(s) immunocytochemistry: suitable, immunoprecipitation (IP): suitable, indirect ELISA: suitable, western blot: 1:20,000 using extracts from 293T (human embryonic kidney) cells expressing a recombinant HA-fusion protein | technique(s) immunoprecipitation (IP): 2.5-4 μg/test using HA-tagged fusion protein from cell lysates, indirect immunofluorescence: 10-20 μg/mL using HA-tagged fusion protein transfected cells, western blot: 0.5-0.8 μg/mL using HA-tagged fusion protein transfected cell extracts |
| form buffered aqueous solution | form buffered aqueous solution | form - | form buffered aqueous solution |
Still not finding the right product?
Probieren Sie unser Produkt-Auswahlhilfe, um Ihre Auswahl einzugrenzen
Lagerklasse
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
-
Where is the biotin located on the IgG? Additionally, what protocol or method did you follow to biotinylate it?
1 answer-
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
What is the isotype and light chain for Monoclonal Anti-HA antibody clone HA-7 produced in mouse?
1 answer-
Monoclonal Anti-HA antibody clone HA-7 is mouse IgG1, kappa light chain.
Helpful?
-