A5713

Monoklonaler Anti-polyhistidin-Antikörper

clone HIS-1, purified immunoglobulin, PBS suspension, suitable for purification of HIS tagged recombinant proteins

• Synonym(e): Monoklonales 6-His-Epitop-Tag, Monoklonales 6xHis-Tag, Monoklonales HHHHHH-Epitop-Tag, Monoklonales Hexahistidin-Tag, Monoklonales His-Tag, Monoklonales His6-Tag, Monoklonales Histidin-Tag, Monoklonales Polyhistidin-Tag
• NACRES: NA.56
• UNSPSC Code: 12352203
• Conjugate: agarose conjugate
• Clone: HIS-1, monoclonal
• Application: —
• Citations: 2

Eigenschaften

• biological source: mouse
• conjugate: agarose conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: HIS-1, monoclonal
• form: PBS suspension
• isotype: IgG2a
• capacity: 15-30 nmol/mL binding capacity
• suitability: suitable for purification of HIS tagged recombinant proteins
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Beschreibung

General description

This mouse IgG2a monoclonal recombinant antibody recognizes native and denatured, reduced forms of synthetic polyhistidine and polyhistidine tagged fusion proteins.

Immunogen

Rekombinantes, mit Polyhistidin markiertes Fusionsprotein.

Application

Antibody suitable for immunoprecipitation and immunoaffinity purification of polyhistidine and polyhistidine tagged fusion proteins from bacteria, bacterial lysates, cells and tissues transfected with polyhistidine fusion protein expressing vectors

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Biochem/physiol Actions

Der Antikörper erkennt synthetisches sowie natives oder denaturiertes Polyhistidin und reduzierte Formen von mit 6X Histidinen markierten Proteinen, die in ausgewählten Vektoren exprimiert werden.

Physical form

Suspension in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Preparation Note

Prepared using Protein A purified antibody coupled to cyanogen bromide-activated agarose at 2.0-2.4 mg antibody per mL resin volume.

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable