Fortfahren mit
A3422
Achromopeptidase aus Bakterien
partially purified powder, ≥20,000 units/mg solid
Synonym(e):
ACP
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
25000 units | Warenkorb auf Verfügbarkeit prüfen | € 528,00 |
50000 units | Warenkorb auf Verfügbarkeit prüfen | € 973,00 |
Über diesen Artikel
Specific activity:
≥20,000 units/mg solid
€ 528,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenform
partially purified powder
Quality Segment
specific activity
≥20,000 units/mg solid
mol wt
27 kDa
foreign activity
Collagenase, present
storage temp.
−20°C
Application
Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections[1]. Product A3422 is from bacteria and is a partially purified powder containing lactose.
Achromopeptidase from bacteria may be used:
- in the homogenization step for genomic DNA extraction from microbial biofilm
- in the lysis of gram-positive strains(
- for DNA extraction from Nocardia strains
Biochem/physiol Actions
Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD600 = 0.6 over 2 hours at 37 °C.
Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family. It is effective for the lysis of gram positive bacteria compared to lysozyme. Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.
Physical form
Partially purified powder.
Other Notes
One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.
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Dieser Artikel | |||
|---|---|---|---|
| specific activity ≥20,000 units/mg solid | specific activity ≥1,000 units/mg solid | specific activity ≥40 units/mg protein (biuret) | specific activity ≥300,000 units/g solid |
| form partially purified powder | form lyophilized powder | form partially purified powder | form partially purified powder |
| mol wt 27 kDa | mol wt 27 kDa | mol wt - | mol wt - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| foreign activity Collagenase, present | foreign activity - | foreign activity - | foreign activity - |
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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