A3422

Achromopeptidase aus Bakterien

partially purified powder, ≥20,000 units/mg solid

• Synonym(e): ACP
• EC-Nummer: 3.4.21.50 (BRENDA, IUBMB)
• MDL-Nummer: MFCD00130333
• UNSPSC-Code: 12352204
• NACRES: NA.54

Eigenschaften

• Form: partially purified powder
• Qualitätsniveau: 200
• Spezifische Aktivität: ≥20,000 units/mg solid
• Mol-Gew.: 27 kDa
• Fremdaktivität: Collagenase, present
• Lagertemp.: −20°C

Beschreibung

Anwendung

Achromopeptidase from bacteria may be used:

• in the homogenization step for genomic DNA extraction from microbial biofilm[1]
• in the lysis of gram-positive strains([2]
• for DNA extraction from Nocardia strains[3]

Achromopeptidase is useful for lysis of Gram-positive bacteria that are resistant to lysozyme. Achromopeptidase, from Sigma, has been used for the isolation and molecular characterization of skin and soft-tissue bacterial infections[4]. Product A3422 is from bacteria and is a partially purified powder containing lactose.

Biochem./physiol. Wirkung

Achromopeptidase is a bacteriolytic enzyme and belongs to serine protease family.[5] It is effective for the lysis of gram positive bacteria compared to lysozyme.[6] Achromopeptidase activity is inhibited in the presence of heavy metals. Achromopeptidase based inhibition assay may be useful for screening the presence of heavy metals.[5]

Achromopeptidase is a lysyl endopeptidase that has a molecular weight of approximately 27 kDa. It works best between pH 8.5 -9. Approximately 500-1,500 μn/ml achromopetidase can be used to lyse cells at a density of OD₆₀₀ = 0.6 over 2 hours at 37 °C.

Einheitendefinition

One unit will lyse 0.4 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 8.0 at 37 °C.

Physikalische Form

Partially purified powder.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P342 + P311
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Faceshields, Gloves