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A2792

Acrylamid/Bis-acrylamid

BioReagent, Molecular Biology, 29:1 (ratio)

Synonym(e):

Acrylamide/Bisacrylamide, 29:1 (3.3% crosslinker), Polyacrylamide solution

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Über diesen Artikel

NACRES:
NA.25
PubChem Substance ID:
UNSPSC Code:
41105319
MDL number:
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grade

Molecular Biology

Quality Segment

product line

BioReagent

form

powder

feed ratio

29:1 (ratio)

foreign activity

DNase, RNase and protease, none detected

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

General description

Acrylamide (AA) is a neurotoxin and carcinogen. It was found in potato products like, french fries and potato chips and also in several cereal-based products. This highly reactive organic compound has the ability to polymerize to form polyacrylamide.

Application

Pulvermischungen für die Rekonstitution sind exakt vorgemischt, um eine 40%ige (w / v) Stammlösung für die Protein-und Nukleinsäure-Elektrophorese zu ergeben. Die Konzentration basiert auf dem Gesamtgewicht von Acrylamid und Bisacrylamid.
Acrylamide/Bis-acrylamide has been used in the preparation of polyacrylamide (PA) gels. It has also been used as a component in the preparation of blotting gel precursor solution, separation gel precursor solution and loading gel precursor solution.

Features and Benefits

Eliminiert die Notwendigkeit toxisches Acrylamid und Bisacrylamid abzuwiegen.

Preparation Note

Geben Sie langsam die angegebene Menge an destilliertem Wasser in den Behälter und lösen den Inhalt.
To make up 100 ml of A2792, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

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Dieser Artikel
A6050A2917A9926
form

powder

form

powder

form

powder

form

liquid

grade

Molecular Biology

grade

Molecular Biology

grade

Molecular Biology

grade

-

feed ratio

29:1 (ratio)

feed ratio

37:1 (ratio)

feed ratio

19:1 (ratio)

feed ratio

19:1

foreign activity

DNase, RNase and protease, none detected

foreign activity

DNase, RNase and protease, none detected

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

foreign activity

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

BioReagent


Still not finding the right product?

Explore all of our products under Acrylamid/Bis-acrylamid


pictograms

Skull and crossbonesHealth hazard

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Lagerklasse

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges


Zulassungslistungen

Zulassungslistungen werden hauptsächlich für chemische Produkte erstellt. Für nicht-chemische Produkte können hier nur begrenzte Angaben gemacht werden. Kein Eintrag bedeutet, dass keine der Komponenten gelistet ist. Es liegt in der Verantwortung des Benutzers, die sichere und legale Verwendung des Produkts zu gewährleisten.

79-06-1

CAS No.

79-06-1

CAS No.


Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  
  1. What is the initial weight of the powder

    1 answer
    1. The initial weight of the powder is 40 g.

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. I purchased the item A2792-100ML, however, there are no instructions on how to reconstitute this so that I have a 30% acrylamide/bis solution. Where can I find instructions?

    1 answer
    1. The instructions on how to use Product A2792 to make 100 mL of a 40 percent solution are provided on the Product Detail Page in the DESCRIPTION section under the heading of Reconstitution:

      "To make up 100 ml of A2792, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution."

      To use this 100 mL of the 40% solution to in turn make a 30% solution, add additional distilled or deionized water to bring the total volume to 133 mL; the total volume can be measured in a graduated cylinder. It should take roughly 30 mL of additional water, but the total volume (133 mL) needs to be measured accurately.
      .
      Alternatively, it be more convenient to order the Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 29:1, which we list as Product A3574. Please see the link below to review this product:
      https://www.sigmaaldrich.com/product/sigma/a3574.

      Helpful?

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