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A0512

Amylose aus Kartoffeln

used as amylase substrate

Synonym(e):

α-Amylose

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PackungsgrößeSKUVerfügbarkeitPreis
250 mg
Warenkorb auf Verfügbarkeit prüfen
€ 56,10
1 g
Warenkorb auf Verfügbarkeit prüfen
€ 97,00
5 g
Warenkorb auf Verfügbarkeit prüfen
€ 464,00
25 g
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€ 2.320,00

Über diesen Artikel

Lineare Formel:
(C6H10O5)n
CAS-Nummer:
UNSPSC Code:
12352201
PubChem Substance ID:
NACRES:
NA.25
MDL number:

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biological source

potato

Quality Segment

form

powder

impurities

amylopectin, essentially free, ≤10% ethanol, ≤2% butanol

color

white to off-white

solubility

0.05 M NaOH: 1 mg/mL, clear to slightly hazy, colorless

storage temp.

room temp

SMILES string

O1[C@@H]([C@@H]([C@H]([C@@H]([C@H]1CO)O[C@H]3O[C@@H]([C@H]([C@@H]([C@H]3O)O)O)CO)O)O)O[C@@H]2[C@H](O[C@@H]([C@@H]([C@H]2O)O)O)CO

InChI

1S/C18H32O16/c19-1-4-7(22)8(23)12(27)17(31-4)34-15-6(3-21)32-18(13(28)10(15)25)33-14-5(2-20)30-16(29)11(26)9(14)24/h4-29H,1-3H2/t4-,5-,6-,7-,8+,9-,10-,11-,12-,13-,14-,15-,16+,17-,18-/m1/s1

InChI key

FYGDTMLNYKFZSV-PXXRMHSHSA-N

General description

A substrate for serum amylase.
Amylose, a ubiquitous carbohydrate polymer in starch, is made up of a long α-1,4-linked linear chain with rare α-1,6-linked branch points. It is usually seen in the amorphous regions of the granule. It has a left-handed α-helical structure with one turn of the helix consisting of six to eight anhydroglucose units.

Application

Amylose from potatoes has been used as a standard to determine the apparent amylose content (AAC) of rice starch by the iodine-binding colorimetric method.

Biochem/physiol Actions

Amylose is a polyglucose polysaccharide that makes starch(s) together with amylopectin. The glucosidic bonds of amylopectin are α-1,4 linkages. Amylose is used to identify, differentiate and characterize amylase(s). Amylose is used as a thickening and gelling agent, and emulsion stabilizer. Amylose is used to prepare films and membranes with potential use in drug delivery.

Other Notes

To gain a comprehensive understanding of our extensive range of Polysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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Dieser Artikel
A8781A6237A7005
biological source

potato

biological source

-

biological source

-

biological source

sweet potato

solubility

0.05 M NaOH: 1 mg/mL, clear to slightly hazy, colorless

solubility

-

solubility

-

solubility

-

Quality Level

300

Quality Level

100

Quality Level

200

Quality Level

200

form

powder

form

powder

form

lyophilized powder

form

ammonium sulfate suspension

storage temp.

room temp

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

impurities

amylopectin, essentially free, ≤2% butanol, ≤10% ethanol

impurities

-

impurities

-

impurities

-


signalword

Warning

pictograms

Exclamation mark

hcodes

Hazard Classifications

Eye Irrit. 2

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Artikel

Explore how carbohydrates function as excipients and carriers in drug delivery, with insights into mechanisms, case studies, and future trends.

Discover the role of carbohydrates in drug delivery, from solubility and stability improvement to advanced controlled-release systems.






Global Trade Item Number

SKUGTIN
A0512-250MG04061833334027
A0512-1G04061835365944
A0512-5G04061833334041
A0512-25G04061833334034

Questions

1–9 of 9 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  3. What is the purity of amylose of Product A0512?

    1 answer
    1. This product is tested for suitability as an amylase substrate. The purity is not determined. Note that this material may contain up to 10% ethanol and 2% butanol. This information is reported on a lot to lot basis. Please see the link below to review a sample or lot specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/sigma/a0512#product-documentation

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. What do the "Types" of Amylose from Potato, Product A0512, mean?

    1 answer
    1. The "Type" of the product has no significant meaning outside Sigma-Aldrich. The differences between products were designated by types before Sigma had established product numbers for specific products. The types were differentiated on several bases, including manufacturing method, source, and purity.

      Helpful?

  6. What is the molecular weight of Amylose from Potato, Product A0512?

    1 answer
    1. Sigma-Aldrich has not determined the molecular weight of the product. One reported range is from 40,000 to 340,000 Daltons, with chains containing 250 to 2000 anhydroglucose units. Source: WHO Food Additives Series No. 5.

      Helpful?

  7. What is the method of preparation of Amylose from Potato, Product A0512?

    1 answer
    1. The method of preparation is given in Street and Close, Clin. Chim. Acta, 1, 256-268 (1956). Potato starch is dissolved in boiling 2% (w/v) NaCl solution, filtered while hot, then cooled. Thymol is added, and the mixture is stirred for 48 hr. Amylopectin is decanted away in the supernatant. The precipitate is washed six times with concentrated thymol solution, four times with absolute ethanol, and dried.

      Helpful?

  8. What is the blue value of Amylose from Potato, Product A0512? How is it determined?

    1 answer
    1. Prepare iodine solution and Solution A:  1. Iodine solution: 0.2% iodine in 2% potassium iodide.2. Solution A: To 100 mg of sample in 100 mL flask, add 1 mL ethanol. Add 10 mL water and 2 mL of 10% NaOH. Warm in boiling water bath until sample dissolves. After cooling, dilute to 100 mL with water.Transfer 2.5 mL of solution A to 250 mL flask. To this flask, add 50 mL water, then 2 drops of 6 N hydrochloric acid, followed by 2.5 mL of Iodine solution. Dilute to 250 mL with water. Read absorbance at 680 nm against a blank.The final solution to be read will be at a sample concentration of 10 μg per mL, and 1% of the iodine solution.This procedure is based on McCready, R. M., and Hassid, W. Z., The Separation and Quantitative Estimation of Amylose and Amyolpectin in Potato Starch. J. Am. Chem. Soc., Vol. 65, pp. 1154-1157 (1943).

      Helpful?

  9. What can I use to solubilize Amylose from Potato, Product A0512?

    1 answer
    1. This product is soluble in 50 mM NaOH at a concentration of 1 mg/mL. The product can also be dissolved as follows (final concentration = 1 mg/ml): Dissolve 100 mg in 1 mL ethanol. Add 10 mL water and 2 mL of 10% NaOH. Heat in boiling water bath until sample dissolves, cool to ambient temperature, then dilute to 100 mL with water.

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