The solubility of this enzyme is tested on the finished product at 1 mL per vial in water. The 100-unit package size will be readily soluble in 1 mL, however, the number of actual units per vial should be reviewed in the lot-specific certificate of analysis. The vial content is typically greater than 100 units. The reconstitution volume would have to be adjusted accordingly in order to achieve a 100 unit per milliliter concentration.
See the link below to review the sample Certificate of analysis:
https://www.sigmaaldrich.com/certificates/sapfs/PROD/sap/certificate_pdfs/COFA/Q14/A6237-100UN0000174833.pdf
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A6237
Apyrase from potato
recombinant, expressed in Pichia pastoris, ATPase ≥1000 units/mg protein, lyophilized powder
Synonym(e):
Apyrase from potato, Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
100 units | Warenkorb auf Verfügbarkeit prüfen | € 141,00 |
500 units | Warenkorb auf Verfügbarkeit prüfen | € 516,00 |
Über diesen Artikel
Recombinant:
expressed in Pichia pastoris
€ 141,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenrecombinant
expressed in Pichia pastoris
Quality Segment
form
lyophilized powder
ATPase activity
≥1000 units/mg protein
shipped in
wet ice
storage temp.
−20°C
General description
Apyrase, also known as ATP-diphosphohydrolase, is expressed in wide variety of plant and animal tissues. The commercially accessible apyrase enzyme is isolated from potato tubers.
Biochem/physiol Actions
Apyrase hydrolyses the phosphoanhydride bonds of nucleoside tri- and di-phosphates in the presence of divalent cations. It has wide substrate specificity for nucleotides. This property of the enzyme makes it suitable for different biotechnical applications, including DNA sequencing and platelet-aggregation inhibition.
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Dieser Artikel | |||
|---|---|---|---|
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| recombinant expressed in Pichia pastoris | recombinant - | recombinant - | recombinant - |
| shipped in wet ice | shipped in wet ice | shipped in - | shipped in - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level - | Quality Level 200 | Quality Level 300 |
| ATPase activity ≥1000 units/mg protein | ATPase activity ≥600 units/mg protein | ATPase activity ≥200 units/mg protein | ATPase activity ≥3.0 units/mg protein |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Hello. Is the concentration of 100 units in 1 mL of water? If not, how can I make the dilution to obtain 100 units? Thanks for you attention! Guilherme
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