91228

Trichloressigsäure

BioUltra, ≥99.5% (T)

• Synonym(e): TCA
• Lineare Formel: Cl₃CCOOH
• CAS-Nummer: 76-03-9
• Molekulargewicht: 163.39
• UNSPSC Code: 12352101
• NACRES: NA.25
• PubChem Substance ID: 57653428
• EC Number: 200-927-2
• Beilstein/REAXYS Number: 970119
• MDL number: MFCD00004177
• Assay: ≥99.5% (T)
• Form: crystals, flakes
• Grade: biotech. grade

Eigenschaften

• grade: biotech. grade
• Quality Level: 200
• vapor density: <1 (vs air)
• vapor pressure: 1 mmHg ( 51 °C)
• product line: BioUltra
• assay: ≥99.5% (T)
• form: crystals, flakes
• impurities: insoluble matter, passes filter test
• ign. residue: ≤0.05%
• refractive index: n20/D 1.62 (lit.)
• pH: <1.0 (25 °C, 0.5 M in H₂O)
• bp: 196 °C (lit.)
• mp: 54-58 °C (lit.)
• solubility: H₂O: 0.5 M at 20 °C, clear, colorless
• density: 1.62 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤30 mg/kg, nitrate (NO₃^-): ≤20 mg/kg, phosphate (PO₄^3-): ≤10 mg/kg, sulfate (SO₄^2-): ≤100 mg/kg
• SMILES string: OC(=O)C(Cl)(Cl)Cl
• cation traces: Al: ≤5 mg/kg, As: ≤0.1 mg/kg, Ba: ≤5 mg/kg, Bi: ≤5 mg/kg, Ca: ≤10 mg/kg, Cd: ≤5 mg/kg, Co: ≤5 mg/kg, Cr: ≤5 mg/kg, Cu: ≤5 mg/kg, Fe: ≤5 mg/kg, K: ≤50 mg/kg, Li: ≤5 mg/kg, Mg: ≤5 mg/kg, Mn: ≤5 mg/kg, Mo: ≤5 mg/kg, Na: ≤50 mg/kg, Ni: ≤5 mg/kg, Pb: ≤5 mg/kg, Sr: ≤5 mg/kg, Zn: ≤5 mg/kg
• λ: 0.5 M in H₂O
• UV absorption: λ: 260 nm A_max: 1.60, λ: 280 nm A_max: 0.15
• InChI: 1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)
• InChI key: YNJBWRMUSHSURL-UHFFFAOYSA-N

Beschreibung

General description

Trichloroacetic acid (TCA) is derived from Trichloroethylene (TCE) metabolism.[1] It is used as an acid decalcifying agent. TCA is used as a fixative for nuclear staining and protein precipitation.

Application

Traditionell zur Fällung von Proteinen verwendet. Zur Bestimmung der Proteinkonzentration durch quantitative Fällung. Zum Entkalken und als Fixationsmittel in der Mikroskopie verwendet.

Trichloroacetic acid (TCA) has been used:

• to determine its effect on avian embryos during an exposure window encompassing cardiac specification
• as a cell fixative
• to terminate the O-acetylserine (thiol)lyase (OASTL) enzymatic assay
• for the precipitation of acidic ribosomal proteins (RPs)

Other Notes

Concentration of protein by quantitative precipitation; Cytochemical demonstration of DNA with a thiazine dye.

Sicherheitshinweise

• pictograms: GHS05,GHS09
• signalword: Danger
• hcodes: H314,H410
• pcodes: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
• wgk: WGK 2
• flash_point_f: >235.4 °F - closed cup
• flash_point_c: > 113 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges
• Lagerklasse: 8A - Combustible corrosive hazardous materials