76216

Papain aus Carica papaya

solution, light brown, ≥10 U/mg protein (~25 mg/ml)

• CAS-Nummer: 9001-73-4
• EC-Nummer: 3.4.22.2 (BRENDA, IUBMB)
• EG-Nummer: 232-627-2
• MDL-Nummer: MFCD00131791
• UNSPSC-Code: 12352204
• eCl@ss: 42030311
• NACRES: NA.54

Eigenschaften

• Biologische Quelle: papaya
• Form: solution
• Spezifische Aktivität: ≥10 U/mg protein (~25 mg/ml)
• Mol-Gew.: M_r ~23000
• Methode(n): cell culture | mammalian: suitable; single cell analysis: suitable
• Farbe: light brown
• Lagertemp.: 2-8°C

Beschreibung

Allgemeine Beschreibung

Papain is a proteolytic enzyme and is naturally occurring in papaya (Carica papaya L.). It is obtained from the latex of cut unripe papaya. Papain belongs to the cysteine proteinase family. It is a globular, single-chain protein composed of four disulfide bridges and catalytically essential residues. Papain consists of an all α-domain and anti-parallel β-sheet domain.

Anwendung

Papain wird als Fleischzartmacher eingesetzt. Es wird außerdem zur Auflösung von Geweben bei der Herstellung von Primärzellkulturen verwendet.

Papain from Carica papaya has been used:

• in the enzymatic dissociation of rat cortices to extract astrocytes for cell culture
• to study its effects on Alpaca seminal plasma viscosity and sperm function
• to study its effects on the yield and cryo-survival of Rhinoceros spermatozoa

Biochem./physiol. Wirkung

Papain ist eine Cysteinprotease, die Peptidbindungen und bekanntermaßen auch das Fc-Fragment von Antikörpern spaltet.

Papain plays an important role in many biological processes in many living organisms. It exhibits proteolytic activity against proteins, amino-acid esters and amide links, and short-chain peptides. Papain preferentially cleaves the peptide bonds of basic amino acids such as arginine, phenylalanine, and lysine. It has a wide range of applications in drug discovery, pharmaceutical, food, baking, and dairy industries.

Einheitendefinition

1 U entspricht der Enzymmenge, die 1 μmol N-Benzoyl-L-arginin-ethylester (BAEE, Fluka^®-Nr. 12880) pro Minute bei pH 6.2 und 25°C hydrolysiert

Sicherheitshinweise

• Piktogramme: GHS08,GHS07
• Signalwort: Danger
• H-Sätze: H315,H319,H334,H335
• P-Sätze: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Gefahreneinstufungen: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Zielorgane: Respiratory system
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 1
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: dust mask type N95 (US), Eyeshields, Faceshields, Gloves