Fluo-3

Name: Fluo-3
Brand: SIGMA

suitable for fluorescence, ~70%

Synonym(e):

4-(2,7-Dichlor-6-hydroxy-3-oxo-9-xanthenyl)-4′-methyl-2,2′-(ethylendioxy)-dianilin-N,N,N′,N′-tetraessigsäure

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Über diesen Artikel

Empirische Formel (Hill-System):

C₃₆H₃₀Cl₂N₂O₁₃

CAS-Nummer:

123632-39-3

Molekulargewicht:

769.54

NACRES:

NA.32

PubChem Substance ID:

329757162

UNSPSC Code:

12352106

MDL number:

MFCD00083337

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Unterstützung erhalten

assay

~70%

Quality Level

100

form

solid

solubility

H₂O: soluble

fluorescence

λ_ex 506 nm; λ_em 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca²⁺

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

Cc1ccc(N(CC(O)=O)CC(O)=O)c(OCCOc2cc(ccc2N(CC(O)=O)CC(O)=O)C3=C4C=C(Cl)C(=O)C=C4Oc5cc(O)c(Cl)cc35)c1

InChI

1S/C36H30Cl2N2O13/c1-18-2-4-24(39(14-32(43)44)15-33(45)46)30(8-18)51-6-7-52-31-9-19(3-5-25(31)40(16-34(47)48)17-35(49)50)36-20-10-22(37)26(41)12-28(20)53-29-13-27(42)23(38)11-21(29)36/h2-5,8-13,41H,6-7,14-17H2,1H3,(H,43,44)(H,45,46)(H,47,48)(H,49,50)

InChI key

OZLGRUXZXMRXGP-UHFFFAOYSA-N

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Dieser Artikel	73881	M043100	927082
Sigma-Aldrich 46393 Fluo-3	Sigma-Aldrich 73881 Fluo 3-AM	Sigma-Aldrich M043100 6-Hexachloro-Fluorescein Phosphoramidite	Sigma-Aldrich 927082 Ni(COD)(Cy-BQ)
suitability suitable for fluorescence	suitability -	suitability -	suitability -
fluorescence λ_ex 506 nm; λ_em 526 nm in 0.1 M Tris pH 8.0, esterase 30 mM Ca²⁺	fluorescence λ_ex 506 nm; λ_em 525 nm in 0.1 M Tris pH 8.0, esterase	fluorescence -	fluorescence -
solubility H₂O: soluble	solubility -	solubility -	solubility -
form solid	form powder	form -	form powder
assay ~70%	assay ≥90% (TLC)	assay ≥97% (³¹P-NMR), ≥97.0% (reversed phase HPLC)	assay ≥95%
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. 2-8°C

Application

Fluo-3 and related molecule Fluo3/AM are used as a fluorescence indicator of intracellular calcium (Ca2+). Fluo-3 may be use for flow cytometry and confocal laser scanning microscopy using visible light excitation (compatible with argon laser sources operating at 488 nm). Fluorescence intensity increases about 40-fold after calcium binding.

Other Notes

Fluoreszenzindikator für Calcium mit Anwendung in der Durchflusszytometrie. Die Fluoreszenzintensität steigt circa um den Faktor 40 nach Bindung an Calcium[1][2]

Lagerklasse

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Flow cytometric measurement of cytoplasmic free calcium in human peripheral blood T lymphocytes with fluo-3, a new fluorescent calcium indicator.

P A Vandenberghe et al.

Journal of immunological methods, 127(2), 197-205 (1990-03-09)

Cytoplasmic free calcium [( Ca2+]i) is a key intracellular messenger in many cell types. We have used fluo-3, a recently developed calcium probe, to study [Ca2+]i in resting and stimulated human peripheral blood T lymphocytes. The spectral properties of fluo-3

All-trans-retinoic acid activated mast cells via Mas-related G-protein-coupled receptor-X2 in retinoid dermatitis.

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Retinoic acid (RA)-induced dermatitis is the most frequent side-effect limiting its widespread use. However, the exact mechanisms triggering dermatitis are not fully understood, including the role of skin mast cells. The newly discovered Mas-related G-protein-coupled receptor-X2 (MRGPRX2) in mast cells

Calcium dysregulation mediates mitochondrial and neurite outgrowth abnormalities in SOD2 deficient embryonic cerebral cortical neurons.

Qijin Zhao et al.

Cell death and differentiation, 26(9), 1600-1614 (2018-11-06)

Mitochondrial superoxide dismutase 2 (SOD2) is a major antioxidant defense enzyme. Here we provide evidence that SOD2 plays critical roles in maintaining calcium homeostasis in newly generated embryonic cerebral cortical neurons, which is essential for normal mitochondrial function and subcellular

Dual effects of gossypol on human hepatocellular carcinoma via endoplasmic reticulum stress and autophagy.

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The international journal of biochemistry & cell biology, 113, 48-57 (2019-05-28)

Treatment outcomes for hepatocellular carcinoma (HCC) remain unsatisfactory, and effective new therapeutic methods are urgently needed. Gossypol has been shown to have an anti-HCC effect, but the underlying mechanism requires further study. In this study, we found gossypol inhibited HCC

Xyloketal B exerts antihypertensive effect in renovascular hypertensive rats via the NO-sGC-cGMP pathway and calcium signaling.

Li-Yan Zhao et al.

Acta pharmacologica Sinica, 39(5), 875-884 (2018-03-30)

Xyloketal B (Xyl-B) is a novel marine compound isolated from mangrove fungus Xylaria sp. (No 2508). We previously showed that Xyl-B promoted endothelial NO release and protected against atherosclerosis through the Akt/eNOS pathway. Vascular NO production regulates vasoconstriction in central

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Global Trade Item Number

SKU	GTIN
46393-1MG-F	04061826678848
46393-5MG-F	04061833277256

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