Consult the Butyrate Esterase Test published in the Clinical Microbiology Procedures Handbook. Alternatively, refer to "A Methodology for Detection and Quantification of Esterase Activity" published in Methods in Molecular Biology.
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4-Methylumbelliferylbutyrat
lipase substrate; esterase substrate, ≥95% (HPCE), powder or crystals, suitable for fluorescence
Synonym(e):
4-Methylumbelliferyl-butyrat
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 1 g | Warenkorb auf Verfügbarkeit prüfen | € 25,50 | |
| 5 g | Warenkorb auf Verfügbarkeit prüfen | € 78,30 |
Über diesen Artikel
Empirische Formel (Hill-System):
C14H14O4
CAS-Nummer:
Molekulargewicht:
246.26
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352204
EC Number:
241-694-7
MDL number:
Beilstein/REAXYS Number:
227973
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Unterstützung erhaltenProduktname
4-Methylumbelliferylbutyrat, suitable for fluorescence, ≥95% (HPCE)
Quality Level
assay
≥95% (HPCE)
form
powder or crystals
mp
90-92 °C (lit.)
solubility
DMF: soluble, DMSO: soluble
fluorescence
λex 312 nm in methanol, λex 360 nm; λem 449 nm (Reaction product), λex 365 nm; λem 445 nm in 0.1 M Tris pH 8.0 (Lipase)
suitability
suitable for fluorescence
storage temp.
−20°C
SMILES string
CCCC(=O)Oc1ccc2C(C)=CC(=O)Oc2c1
InChI
1S/C14H14O4/c1-3-4-13(15)17-10-5-6-11-9(2)7-14(16)18-12(11)8-10/h5-8H,3-4H2,1-2H3
InChI key
WKPUJZVCZXWKCK-UHFFFAOYSA-N
Application
geeignet als fluorogenes Substrat für Esterasen/Lipasen
Other Notes
Fluorogenic substrate for butyrate esterase[1]
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Dieser Artikel | |||
|---|---|---|---|
| assay ≥95% (HPCE) | assay ≥95% (HPCE) | assay ≥98% (TLC) | assay ≥98% (TLC) |
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability - | suitability - |
| solubility DMF: soluble, DMSO: soluble | solubility 2-methoxyethanol: soluble, DMSO: soluble, chloroform: soluble | solubility chloroform: 100 mg/mL, clear, colorless | solubility water: 50 mg/mL, clear, colorless to very faintly yellow |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| form powder or crystals | form solid | form powder | form powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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M Vaneechoutte et al.
Journal of clinical microbiology, 26(6), 1227-1228 (1988-06-01)
Branhamella catarrhalis can be distinguished from Neisseria spp. by the presence of butyrate esterase. This enzyme can be rapidly detected when 4-methylumbelliferyl butyrate is used as the substrate. All B. catarrhalis strains tested gave a positive fluorescence reaction within 5
Neda Gilannejad et al.
Animals : an open access journal from MDPI, 10(5) (2020-05-24)
Thick-lipped grey mullet (Chelon labrosus) is a candidate for sustainable aquaculture due to its omnivorous/detritivorous feeding habit. This work aimed to evaluate its digestive and growth potentials from larval to early juvenile stages. To attain these objectives the activity of
Ivan Kurtovic et al.
Fish physiology and biochemistry, 36(4), 1041-1060 (2010-02-10)
Lipases were purified from delipidated pyloric ceca powder of two New Zealand-sourced fish, Chinook salmon (Oncorhynchus tshawytscha) and hoki (Macruronus novaezelandiae), by fractional precipitation with polyethylene glycol 1000, followed by affinity chromatography using cholate-Affi-Gel 102, and gel filtration on Sephacryl
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 19362-1G | 04061838760869 |
| 19362-5G | 04061838760876 |
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I am looking for a general esterase activity protocol using 4-Methylumbelliferyl butyrate as a substrate.
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