Sodium thiosulfate is used to remove any unreacted silver to prevent increased background staining, per the Instructions For Use. These instructions also specify the suitability of well-prepared tissue sections cut 5–6 microns and fixed in neutral buffered formalin for iron staining, as well as the preparation method for blood or bone marrow films.
Fortfahren mit
SE100
Silber-Verstärker-Kit
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 1 kit | Warenkorb auf Verfügbarkeit prüfen | € 255,00 |
Über diesen Artikel
NACRES:
NA.47
UNSPSC Code:
12171500
Technischer Dienst
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Unterstützung erhaltenform
liquid
Quality Level
usage
sufficient for ≥100 slides staining and fixing
dilution
(for the determine the presence of gold nanorods in various tissues)
application(s)
diagnostic assay manufacturing
hematology
histology
storage temp.
2-8°C
Application
Silver Enhancer Kit has been used to determine the presence of gold nanorods in various tissues and to prepare silver enhanced gold colloids for electron microscopy.
Biochem/physiol Actions
Lösung A und Lösung B werden unmittelbar vor dem Gebrauch 1:1 gemischt und auf den Objektträger mit dem gold-markierten Abschnitt appliziert. Nach 5-10 Minuten wird der Objektträger mit destilliertem Wasser gewaschen, für 2-3 Minuten in einer Natriumthiosulfat-Lösung fixiert und nochmals gespült. Der Abschnitt ist nun nach Bedarf bereit für die Gegenfärbung und Montage.
Features and Benefits
Colloidal gold labels are normally visible only at the electron microscope level. Silver Enhancer enlarges the gold colloid label by precipitation of metallic silver to give a high contrast signal visible under a light microscope. A darkroom is not necessary for this procedure, as the silver enhancer working solution is light-stable for 20-30 minutes under ordinary laboratory lighting.
Packaging
Das Silberverstärkungskit besteht aus Lösung A (Silbersalz), Lösung B (Initiator), Natriumthiosulfat (Fixiermittel) und einem technischen Datenblatt mit der Beschreibung der Anwendung.
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing | application(s) diagnostic assay manufacturing |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| usage sufficient for ≥100 slides staining and fixing | usage - | usage - | usage - |
| storage temp. 2-8°C | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| dilution (for the determine the presence of gold nanorods in various tissues) | dilution - | dilution - | dilution - |
| form liquid | form - | form powder or crystals | form powder, crystals or granules |
Nur Kit-Komponenten
Produkt-Nr.
Beschreibung
- Sodium thiosulfate pentahydrate, reagent grade, ≥99.5% 500 g
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Repr. 1B
Lagerklasse
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
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Verwandter Inhalt
Instructions
Kathleen Grennan et al.
Talanta, 68(5), 1591-1600 (2008-10-31)
With lower limits of detection and increased stability constantly being demanded of biosensor devices, characterisation of the constituent layers that make up the sensor has become unavoidable, since this is inextricably linked with its performance. This work describe the optimisation
A histological evaluation and in vivo assessment of intratumoral near infrared photothermal nanotherapy-induced tumor regression
Hadiyah N
International journal of nanomedicine (2014)
Using Immuno-Scanning Electron Microscopy
for the Observation of Focal Adhesion-substratum
interactions at the Nano- and Microscale in S-Phase Cells
for the Observation of Focal Adhesion-substratum
interactions at the Nano- and Microscale in S-Phase Cells
Manus J.P. Biggs
Cell Culture Labfax (2011)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| SE100-1KT | 04061832344409 |
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What is the fixer Sodium Thiosulfate in Ag enhancer kit SE100 used for?
1 answer-
Helpful?
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For the Silver Enhancer Kit (Cat # SE100), what is the capping agent on the resulting silver nanoparticle that is formed after the silver enhancement reaction?
1 answer-
Am not sure what is meant by the capping reagent. The Silver Enhancer kit shares similarities with the Grocott Methanamine Silver Nitrate (GMS) procedure for fungus, basement membranes or urates. The GMS and SE100 kit are both silver stain methods. The SE100 has been formulated to stain gold nanoparticles instead of fungi, basement membranes, or urates. The SE100 has 3 reagents. Reagent 1 is a silver nitrate solution. Solution 2 is a Tungstosilicic Acid Hydrate solution. This compound likely replaces the methenamine reagent typically used in the GMS procedure. Gold Chloride is considered a toning solution in the GMS procedure. There is not an equivalent reagent in the SE100 procedure. The third reagent in the SE100 kit is a sodium thiosulfate solution which removes any unreduced silver. It is likely that the Tungstosilicic Acid Hydrate is what you would refer to as a capping agent.
Helpful?
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