93745

TSC-Aagar

NutriSelect^® Basic, according to ISO, powder, selective and differential for Clostridium perfringens, pack of 500 g, suitable for microbiology

• Synonym(e): Tryptose-Sulfit-Cycloserin-Agar
• NACRES: NA.74
• UNSPSC Code: 41171606
• Manufacturer/tradename: NutriSelect^® Basic
• Agency: according to ISO 15213:2003, according to ISO 7937:2004, according to ISO
• Form: powder
• Suitability: selective and differential for Clostridium perfringens
• Application(s): agriculture; clinical testing; environmental; food and beverages; pathogen testing; water monitoring, microbiology

Eigenschaften

• Quality Segment: 200
• agency: according to ISO 15213:2003, according to ISO 7937:2004, according to ISO
• sterility: non-sterile
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L , ammonium ferric citrate, 1 g/L , meat peptone (peptic), 15 g/L , sodium disulfite, 1 g/L , soya peptone, 5 g/L , yeast extract, 5 g/L
• packaging: pack of 500 g
• manufacturer/tradename: NutriSelect^® Basic
• technique(s): microbiological culture: suitable
• final pH: 7.6±0.2 (25 °C)
• application(s): agriculture; clinical testing; environmental; food and beverages; pathogen testing; water monitoring, microbiology
• suitability: selective and differential for Clostridium perfringens

Beschreibung

Application

Für die Isolierung und Auszählung vegetativer Formen oder von Sporen von Clostridium perfingens in Lebensmitteln, klinischen Proben und anderem Material nach Harmon et.al. (1971).

Preparation Note

Base Agar:
Dissolve 42 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes. Cooling to approx. 50°C.

TSC Agar:
To 1 litre Base Agar add 400 mg rehydrated D-cycloserine (Cat. No. 30020) as a sterile filtered aqueous solution or 2 vials of Perfringens T.S.C. Supplement (Cat. No. P9352) and 50 ml Egg Yolk Emulsion (Cat. No. 17148). Mix well and pour into petri plates.

TSC Agar egg yolk free (also used as overlay agar):
Add 400 mg rehydrated D-cycloserine (Cat. No. 30020) or 2 vials of Perfringens T.S.C. Supplement (Cat. No. P9352) to 1 litre Base Agar and mix well.

TSC Fluorescence Agar:
Add to 1 litre Base Agar 5.5 g rehydrated TSC Agar supplement (Cat. No. 80548) as a sterile filtered aqueous solution. Mix well and pour plates.

SFP Agar (Shahidi-Ferguson Perfringens Agar):
To 1 litre Base Agar 2 vials of Perfringens S.F.P. Supplement Cat. No. P9477) and 50 ml Egg Yolk Emulsion (Cat. No. 17148) are added. Mix well and pour into sterile petri plates.

Other Notes

Isolation and enumeration of sulphite-reducing Clostridium spores and Group D faecal streptococci from oysters[1]; Membrane filtration enumeration of faecal clostridia and C. perfringens in water[2]

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

This product will be discontinued in the near future; however, stock is still available. The recommended replacement product is 1.11972 - Tryptose sulfite cycloserine agar (base)

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H319
• pcodes: P264 - P280 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2
• Lagerklasse: 11 - Combustible Solids
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)