SCC115

CH27 Mouse B Cell Lymphoma Cell Line

Mouse

• Synonym(e): CH27 [Mouse lymphoma]
• UNSPSC Code: 41106514
• NACRES: NA.81
• eCl@ss: 32011203
• Biological source: mouse

Eigenschaften

• Produktname: CH27 Mouse B Cell Lymphoma Cell Line, CH27 mouse B cell lymphoma celll line is a useful model for the study of B-cell biology and tumor immunology.
• biological source: mouse
• Quality Level: 100
• shipped in: ambient
• storage temp.: -140 to -196°C

Beschreibung

General description

CH27 Mouse B Cell Lymphoma Cell Line is one of the CH series of mouse B-cell lymphomas that arise from the spleen cells of B10.H-2a-H-4b p/Wts (2a4b) mice, either spontaneously or after transplantation of the spleen cells to syngeneic or F1 hybrid recipients [1] [2] [3].

CH27 cells express surface IgM and Ia antigens [3] along with Ly-1 (CD5) and Ig which bind sheep (SRBC) and chicken erythrocytes and bromelain-treated mouse erythrocytes [2] [3] [4] . The hapten recognized by SRBC-binding CH lymphomas is a phospholipid, either phosphatidylchone or sphingomyelin [5]. Phospholipids are a common component of cell membranes and thus cells expressing immunoglobulin with specificity for SRBC can also be found in the spleens of normal mice [6]. This suggests that CH lymphomas represent neoplastic analogues of Ly-1+ normal B-cells [3].

Application

Research Category
Cancer

Immune Response

Inflammation & Immunology

Subject to local law, this product is intended to be sold for internal in vitro research use only subject to terms and conditions found here: www.sigmaaldrich.com/restrictedcelluse. This product may not be: re-engineered or copied; used to make derivatives, modifications or functional equivalents; used to obtain patents or other IP claiming use of the product; used to develop, test, or manufacturer a commercial product; used as a component in a commercial product; resold or licensed; used in any clinical applications or trials; or used in humans. A license or limited commercial use agreement is required for use by any for-profit entity, use in services, and use in sponsored academic research. For information regarding any such use, please contact [email protected].

Biochem/physiol Actions

Cancer Cells

Preparation Note

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Analysis Note

• Each vial contains ≥ 1X106 viable cells.
• Cells are tested negative for infectious diseases by a Mouse Essential CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of mouse origin and negative for inter-species contamination from rat, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash