OP39

Anti-c-ErbB2/c-Neu (Ab-5) Mouse mAb (TA-1)

liquid, clone TA-1, Calbiochem^®

• Synonym(e): Anti-HER2, Anti-c-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: TA-1, monoclonal
• Species reactivity: human
• Application: —
• Citations: 3

Eigenschaften

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: TA-1, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human
• should not react with: mouse, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Flow Cytometry (5 µg/mL, ; Frozen Sections (5 µg/mL); Immunoblotting ; Immunofluorescence (5 µg/mL, ; Immunoprecipitation (1 µg/sample, ; Neutralization Studies (not recommended and application references))
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... ERBB2(2064)

Beschreibung

General description

Anti-c-ErbB2/c-Neu (Ab-5), mouse monoclonal, clone TA-, recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells. It is validated for FC, WB, IF, IP, and IHC on frozen sections.

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing spleonocytes with SP2/0 mouse myeloma cells (see application references). Recognizes the c-ErbB2/c-neu protein.

Recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells.

Application

Flow Cytometry (5 µg/ml, see application references)

Frozen Sections (5 µg/ml)

Immunoblotting (see application references)

Immunofluorescence (5 µg/ml, see application references)

Immunoprecipitation (1 µg/sample, see application references)

Neutralization Studies (not recommended, see comments and application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM PBS, 0.2% gelatin, pH 7.5.

Analysis Note

Positive Control
SK-BR-3 cells

Other Notes

DiFiore, P.P., et al. 1987. Science237, 178.
Slamon, D.J., et al. 1987. Science235, 177.
Varley, J.M., et al. 1987. Oncogene1, 423.
Yamamoto, T., et al. 1986. Nature319, 230.
Blick, M., et al. 1984. Blood64, 1234.
Schwab, M., et al. 1984 Cold Spring Harbor Laboratory2, 215.
Bargmann, C.I., et al. 1968. Nature319, 226.

Recognizes an extracellular epitope and does not cross react with human EGF-receptor. It will not inhibit in vivo protein tyrosine kinase activity. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable