OP14

Anti-c-ErbB2/c-Neu (Ab-2) Mouse mAb (9G6)

liquid, clone 9G6, Calbiochem^®

• Synonym(e): Anti-ErbB2, Anti-Erythroblastosis Virus, Anti-HER2, Anti-c-Neu
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: 9G6, monoclonal
• Species reactivity: human
• Application: —
• Citations: 1

Eigenschaften

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 9G6, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Frozen Sections (5 µg/mL); Immunoprecipitation (1 µg/sample); Paraffin Sections )
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... ERBB2(2064)

Beschreibung

General description

Anti-c-ErbB2/c-Neu (Ab-2), mouse monoclonal, clone 9G6, recognizes the ~185 kDa c-ErbB2/c-Neu SK-BR-3 cells and breast carcinoma. It is validated for IP and IHC on frozen and paraffin sections.

Recognizes the ~185 kDa c-ErbB2/c-Neu protein in SK-BR-3 cells and breast carcinoma tissue. Does not cross-react with v-ErbB2 or the EGF receptor.

This clone expresses the kappa light chain isotype. Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP2/0 Ag14 myeloma cells. Recognizes the ~185 kDa c-ErbB2/c-neu protein.

Application

Frozen Sections (5 µg/mL)Immunoprecipitation (1 µg/sample)Paraffin Sections

Packaging

Please refer to vial label for lot-specific concentration.

Analysis Note

Negative Control
Normal skin tissue or HepG2 cells

Other Notes

Immunoprecipitates the 185 kDa c-neu protein and has been reported to stain formalin-fixed paraffin-embedded tissue sections of human breast carcinomas overexpressing the c-neu protein (see application references). This antibody reacts with a cell surface epitope of c-neu but does not cross-react with the EGF receptor. In one study ductal carcinomas in situ displaying large-cell, comedo growth type, were stained while none of 16 ductal carcinomas in situ of small-cell, papillary, or cribiform growth type were stained by this antibody. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: nwg