OP13

Anti-N-Myc Mouse mAb (NCM II 100)

liquid, clone NCM II 100, Calbiochem^®

• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: NCM II 100, monoclonal
• Species reactivity: human, mouse
• Application: —
• Citations: 34

Eigenschaften

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: NCM II 100, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: human, mouse
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (Frozen Sections (1-5 µg/mL); Immunoblotting (1-5 µg/mL, ; Immunofluorescence (1-5 µg/mL, ; Immunoprecipitation (1-2 µg/sample, )
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MYCN(4613)

Beschreibung

General description

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with specified immunogen and fusing splenocytes with SP2/0 mouse myeloma cells (see application references). Recognizes N-myc and its cleavage products.

Recognizes the N-myc protein and its cleavage product in IMR5 cells.

This Anti-N-Myc Mouse mAb (NCM II 100) is validated for use in Frozen Sections, Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of N-Myc.

Immunogen

recombinant, human, N-myc fusion protein, expressed in E. coli

Human

Application

Frozen Sections (1-5 µg/ml)

Immunoblotting (1-5 µg/ml, see application references)

Immunofluorescence (1-5 µg/ml, see application references)

Immunoprecipitation (1-2 µg/sample, see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 50 mM sodium phosphate buffer, 50% glycerol.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Positive Control
IMR5 cells

Other Notes

Brondyk, W.H. 1991. Oncogene6, 1269.
Ikegaki, N., et al. 1988. Adv. Neuroblastoma Research.2, 133.
LeGouy, E., et al. 1987. In Nuclear Oncogenes, Cold Spring Harbor Laboratory, 144.
Cole, M.D., 1986. Ann. Rev. Gen.20, 361.
Ikegaki, N., et al. 1986. Proc. Natl. Acad. Sci. USA83, 5929.
Slamon, D.J., et al. 1986. Science232, 768.
Seeger, R.C., et al. 1985. New Engl. J. Med.313, 1111.
Brodeur, G.M., et al. 1984. Science224, 1121.
Michitsch, R.W., et al. 1984. Mol. Cell. Biol.11, 2370.
Kohl, N.E., et al. 1983. Cell35, 603.
Schwab, M., et al. 1983. Nature305, 245.

It is important to compensate for the short half life of this protein when preparing tissue sections or extracts. We recommended that all preparations be kept cold and that a cocktail of protease inhibitors be used. Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 3