GR11

Anti-IGF-IR (Ab-1) Mouse mAb (αIR3)

liquid, clone αIR3, Calbiochem^®

• Synonym(e): Anti-IGF-R, Anti-Insulin-Like Growth Factor Receptor, Anti-Insulin-Like Growth Factor Receptor, Anti-IGF-R
• NACRES: NA.43
• UNSPSC Code: 12352203
• Clone: αIR3, monoclonal
• Species reactivity: human
• Application: —
• Citations: 10

Eigenschaften

• biological source: mouse
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: αIR3, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human
• manufacturer/tradename: Calbiochem^®
• storage condition: do not freeze
• dilution: (Immunoblotting (not recommended); Immunofluorescence (1-5 µg/mL); Immunoprecipitation (1-2 µg/sample); Neutralization Studies (1 µg/mL); Paraffin Sections (not recommended))
• isotype: IgG1
• shipped in: wet ice
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... IGF1R(3480)

Beschreibung

General description

Purified mouse monoclonal antibody generated by immunizing SJL mice with the specified immunogen and fusing splenocytes with FO mouse myeloma cells (see application references). Recognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.

Recognizes the ~130 kDa α and the ~90 kDa β subunits of IGF-I receptor.

This Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) is validated for use in Immunoblotting, IF, IP, Neutralization Studies, Paraffin Sections for the detection of IGF-IR (Ab-1).

Immunogen

Human

partially purified, human placenta IGF-I receptor

Application

Immunoblotting (not recommended)

Immunofluorescence (1-5 µg/ml)

Immunoprecipitation (1-2 µg/sample)

Neutralization Studies (1 µg/ml; use Cat. No. GR11L)

Paraffin Sections (not recommended)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 0.05 M sodium phosphate buffer, 0.2% gelatin.

Analysis Note

Negative Control
HS27 cells

Positive Control
HepG2 cells

Other Notes

Anti-IGF-I Receptor (Ab-1) Mouse mAb (αIR3) immunoprecipitates the α and β subunits of the IGF-I receptor. Blocks IGF-I binding to its receptor and may bind weakly to the insulin receptor. It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in indvidual systems.

Roth, R. 1988. Science239, 1269.
Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm.149, 276.
Rosen, O.M., 1987. Science257, 1452.
Rechler, M.M. and Nissley, S.P., 1986. Hormone Res.24, 152.
Ullrich, A., et al. 1986. EMBO J.5, 2503.
Zapf, S. and Froesch, E.R., 1986. Hormone Res.24, 121.
Humbel, R.E., 1984. I. Chemistry; in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York).
Kull, F.C., et al. 1983. J. Biol. Chem.258, 6561.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable