The sample amount depends on the detection method. The recommended sample amounts are as follows;
CBB staining: 25 µg or more
Fluorescent staining: 25 µg or less
Silver Staining: 10 µg or less
Fluorescent pre-labeling: 3 µg or less
BM-100
Auto2D® 2-D Electrophoresis Device
Automates 2D Gel Electrophoresis from 1st dimension IEF to 2nd dimension SDS-PAGE
Synonym(e):
2D Electrophoresis, 2DE, IEF, SDS-PAGE
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1 PKG
€ 55.380,00
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NACRES:
NC.01
UNSPSC Code:
41100000
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Unterstützung erhaltenProduktname
Auto2D® 2-D Electrophoresis Device, automates 2D gel electrophoresis
product line
Auto2D®
technique(s)
SDS-PAGE: suitable, electrophoresis: suitable, western blot: suitable
storage temp.
room temp
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| storage temp. room temp | storage temp. - | storage temp. room temp | storage temp. - |
| product line Auto2D® | product line Auto2D® | product line Auto2D® | product line Auto2D® |
| technique(s) SDS-PAGE: suitable | technique(s) - | technique(s) - | technique(s) - |
General description
The Auto2D® 2-D Electrophoresis Device automates two-dimensional gel electrophoresis. The applied sample enters the first-dimension gel passively, separating proteins by their isoelectric points. After equilibration, the first-dimension gel is set onto a horizontal SDS-PAGE gel, which resolves proteins by molecular weight.
Two-dimensional gel electrophoresis has generally been regarded as difficult to perform and time-consuming, requireing advanced user training while offering low reproducibility and high inter-operator variability.
Two-dimensional gel electrophoresis has generally been regarded as difficult to perform and time-consuming, requireing advanced user training while offering low reproducibility and high inter-operator variability.
Application
- Differential proteomic analysis
- Post-translational modification
- Protein Isoforms
- Western Blotting
Features and Benefits
Auto2D® 2-D Electrophoresis Device is user-friendly and enable two-dimensional electrophoresis in 1-2 hours for faster, more reproducible results.
- Fully automated to eliminate tedious handling steps
- Fast results in 1-2 hours
- High reproducibility
- Easy to use, with no advanced training required
- Lower inter-operator variability
Preparation Note
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Legal Information
Auto2D is a registered trademark of Merck KGaA, Darmstadt, Germany
Analysenzertifikate (COA)
Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.
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Artikel
Durch das Auto2D® 2-D-Elektrophoresystem werden schwierige 2-D-Elektrophoreseverfahren auf schnelle, einfache und reproduzierbare Weise automatisiert. Lokalisieren Sie mit dem Auto2D®-System schwer auffindbare Proteine in weniger als zwei Stunden bei hoher Reproduzierbarkeit.
The Auto2D® 2-DE system automates difficult 2D gel electrophoresis methods for highly reproducible protein analysis in under 2 hours.
Protokolle
Einführung in PAGE. Erfahren Sie mehr über den Hintergrund der SDS-PAGE und das Protokoll für die Auftrennung von Proteinen nach Größe in einem Polyacrylamidgel.
The Auto2D® Automated 2-D Gel Electrophoresis Device video and detailed protocol provide reliable methods to prepare your samples and operate the Auto2D® Device.
Introduction to PAGE. Learn about SDS-PAGE background and protocol for the separation of proteins based on size in a poly-acrylamide gel.
Verwandter Inhalt
Auto2D® 2-D Gel Electrophoresis Device frequently asked questions for 2-D PAGE and additional 2DE protein separation workflow needs.
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How much is the appropriate sample amount?
1 answer-
Helpful?
-
-
How much is the appropriate sample amount?
1 answer-
The sample amount depends on the detection method. The recommended sample amounts are as follows;
CBB staining: 25 µg or more
Fluorescent staining: 25 µg or less
Silver Staining: 10 µg or less
Fluorescent pre-labeling: 3 µg or lessHelpful?
-
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