268298
DAPI, Dihydrochlorid
Cell-permeable DNA-binding dye.
Synonym(e):
DAPI, Dihydrochlorid, 4ʹ,6-Diamidino-2-phenylindol, 2HCl
Größe auswählen
| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 10 mg | Warenkorb auf Verfügbarkeit prüfen | € 218,00 |
Über diesen Artikel
€ 218,00
Quality Segment
description
RTECS - NL5995050
assay
≥90% (HPLC)
form
solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, desiccated (hygroscopic), protect from light
color
yellow
solubility
water: 5 mg/mL, ethanol: soluble, methanol: soluble
fluorescence
λex ~359 nm, λem ~461 nm
shipped in
ambient
storage temp.
−20°C
SMILES string
[Cl-].[Cl-].[nH]1c2c(cc1c3ccc(cc3)C(=N)N)ccc(c2)C(=N)N.[H+].[H+]
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
InChI key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
General description
Biochem/physiol Actions
Bindet bevorzugt an Adenin- und Thymin-reiche DNA.
Preparation Note
Legal Information
Disclaimer
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| color yellow | color - | color - | color - |
| fluorescence λex ~359 nm, λem ~461 nm | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) |
| form solid | form powder | form powder | form liquid |
| solubility water: 5 mg/mL, methanol: soluble | solubility H2O: 20 mg/mL, PBS: insoluble | solubility H2O: 20 mg/mL, PBS: insoluble | solubility water: soluble |
| Quality Level 100 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
signalword
Warning
hcodes
Hazard Classifications
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
target_organs
Respiratory system
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Verwandter Inhalt
Expression of genetically-encoded fluorescently-tagged proteins has widely been employed for real-time visualization of cellular behavior and trafficking. Prepackaged, ready-to-use, high-titer lentiviral particles (which we have termed “lentiviral biosensors”) encoding GFP- or RFP-tagged proteins are a convenient, robust solution for fluorescent imaging of transduced cells. Compared to other nonviral transfection methods, lentiviral transduction, in many cases, offers higher transfection efficiency and more homogeneous protein expression, particularly for traditionally hard-to-transfect primary cell types. Lentiviral biosensors are ideal for use with fixed and live cell fluorescent microscopy, and are non-disruptive towards cellular function. GFP- or RFP-protein localization matches well with antibody-based immunostaining and demonstrates altered patterns of expression upon treatment with modulators of cell function and phenotype. Lentiviral biosensors provide a broadly effective, convenient method for visualization of cell behavior under a variety of physiological and pathological treatment conditions, in both endpoint and real-time imaging modalities. In this study, we focus on lentiviral biosensors containing GFP-LC3 and RFP-LC3 for the study of autophagosome formation.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| 268298-10MG | 04055977197785 |