If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
D8417
4′,6-Diamidin-2-phenylindol -dihydrochlorid
powder, BioReagent, suitable for cell culture, ≥98% (HPLC), suitable for fluorescence
Synonym(e):
2-(4-Amidinophenyl)-6-indolcarbamidin -dihydrochlorid, DAPI -dihydrochlorid
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€ 56,80
€ 193,00
€ 348,00
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InChI key
FPNZBYLXNYPRLR-UHFFFAOYSA-N
InChI
1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
SMILES string
Cl.Cl.NC(=N)c1ccc(cc1)-c2cc3ccc(cc3[nH]2)C(N)=N
product line
BioReagent
form
powder
technique(s)
cell culture | mammalian: suitable
solubility
H2O: 20 mg/mL, PBS: insoluble
ε (extinction coefficient)
30 at 263 nm in H2O
fluorescence
λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex)
suitability
suitable for fluorescence
storage temp.
2-8°C
Quality Level
assay
≥98% (HPLC)
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Dieser Artikel | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) - | technique(s) - | technique(s) transfection: suitable |
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability - | suitability suitable for fluorescence |
| fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex), λex 374 nm; λem 461 nm in 10 mM Tris; 1 mM EDTA; pH 8.0; DNA | fluorescence λex ~359 nm, λem ~461 nm | fluorescence λex 340 nm; λem 488 nm (nur DAPI), λex 364 nm; λem 454 nm (DAPI-DNA-Komplex) |
| form powder | form powder | form solid | form powder |
| solubility H2O: 20 mg/mL, PBS: insoluble | solubility DMF: soluble, H2O: soluble | solubility water: 5 mg/mL, ethanol: soluble, methanol: soluble | solubility H2O: 20 mg/mL, PBS: insoluble |
| storage temp. 2-8°C | storage temp. room temp | storage temp. −20°C | storage temp. 2-8°C |
General description
Application
Biochem/physiol Actions
Disclaimer
signalword
Warning
hcodes
Hazard Classifications
Skin Irrit. 2 - Skin Sens. 1A - STOT SE 3
target_organs
Respiratory system
Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Instructions
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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