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23-033-M

MDM2 (c-Myc tagged) Protein, active, 10 µg

Active, full length, recombinant human MDM2. N-terminal c-Myc, GST-tagged. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

Synonym(e):

Double minute 2 protein, p53-binding protein Mdm2, Hdm2

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PackungsgrößeSKUVerfügbarkeitPreis
250 μg
Warenkorb auf Verfügbarkeit prüfen
€ 3.900,00

Über diesen Artikel

UNSPSC Code:
12352202
NACRES:
NA.41
eCl@ss:
32160405
Biological source:
human
Recombinant:
expressed in E. coli

€ 3.900,00

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Unterstützung erhalten

biological source

human

Quality Level

100

recombinant

expressed in E. coli

manufacturer/tradename

Upstate®

technique(s)

activity assay: suitable

GenBank accession no.

AF527840

General description

MDM2 is an E3 ubiquitin ligase that regulates the turnover of several cellular factors including the p53 tumour suppressor protein and notably MDM2 itself. The suppression of p53 activity by an amplified Mdm2 gene has been observed in many tumours, including soft tissue sarcomas, osteosarcomas and breast tumours.
N-terminal GST-tagged, recombinant human TRAF6 full length, expressed in E.coli.

Application

Ubiquitination Cascade Comoponent: E3

Physical form

Purified using glutathione sepharose followed by gel filtration.

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

GenBank is a registered trademark of United States Department of Health and Human Services
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Dieser Artikel
MABE340-AF555MABE340-AF48804-1530
MDM2 (c-Myc tagged) Protein, active, 10 µg Active, full length, recombinant human MDM2. N-terminal c-Myc, GST-tagged. For use in Enzyme Assays. Functions as an E3 ligase in ubiquitination assays.

Sigma-Aldrich

23-033-M

MDM2 (c-Myc tagged) Protein, active, 10 µg

Anti-MDM2 Antibody, clone IF2, Alexa Fluor™ 555 Conjugate clone IF2, from mouse, ALEXA FLUOR™ 555

Sigma-Aldrich

MABE340-AF555

Anti-MDM2 Antibody, clone IF2, Alexa Fluor™ 555 Conjugate

Anti-MDM2 Antibody, clone IF2, Alexa Fluor™ 488 Conjugate clone IF2, from mouse, ALEXA FLUOR™ 488

Sigma-Aldrich

MABE340-AF488

Anti-MDM2 Antibody, clone IF2, Alexa Fluor™ 488 Conjugate

Anti-MDM2 Antibody, clone 3G9 clone 3G9, from mouse

Sigma-Aldrich

04-1530

Anti-MDM2 Antibody, clone 3G9

recombinant

expressed in E. coli

recombinant

-

recombinant

-

recombinant

-

biological source

human

biological source

mouse

biological source

mouse

biological source

mouse

technique(s)

activity assay: suitable

technique(s)

immunocytochemistry: suitable

technique(s)

immunocytochemistry: suitable

technique(s)

immunocytochemistry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

Quality Level

100

Quality Level

-

Quality Level

100

Quality Level

100

GenBank accession no.

AF527840

GenBank accession no.

-

GenBank accession no.

-

GenBank accession no.

-

manufacturer/tradename

Upstate®

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H317

Hazard Classifications

Skin Sens. 1

Lagerklasse

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Verwandter Inhalt

Detecting Substrate Ubiquitination by the E3 Ligase, XIAP, Using Quantitative Assays

Quantitative assays for investigating ubiquitination pathways are essential for advancing ubiquitin-related drug discovery research. To illustrate the utility of in vitro ubiquitination enzyme cascades, we quantify the ubiquitination of Smac/DIABLO by the E3 ligase XIAP using a functional enzymatic reaction coupled to multiple assay readouts. We compare different detection methods that can be used to test small molecules for their potential to modulate XIAP-mediated ubiquitination in the context of this assay. The data provides a basis for use of these recombinant E3 ligase cascades in various applications, including screening campaigns.

Global Trade Item Number

SKUGTIN
23-03304053252294785
23-033M04053252294495

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