04-889

Anti-Src Antibody, clone N6L, rabbit monoclonal

culture supernatant, clone N6L, from rabbit

• Synonym(e): proto-oncogene tyrosine-protein kinase SRC, protooncogene SRC, Rous sarcoma, tyrosine kinase pp60c-src, tyrosine-protein kinase SRC-1, v-src avian sarcoma (Schmidt-Ruppin A-2) viral oncogene ; ; homolog, v-src sarcoma (Schmidt-Ruppin A-2) viral onco
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: N6L, monoclonal
• Application: WB
• Citations: 3

Eigenschaften

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: N6L, monoclonal
• species reactivity: human
• technique(s): western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_005408.1
• UniProt accession no.: P12931
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... SRC(6714)

Beschreibung

General description

pp60c-Src is the prototype non-receptor tyrosine kinase. Homology to Src in three regions is shared by a number of proteins: SH1 being the catalytic domain, SH2 being a phospho-tyrosine binding domain, and SH3 being a proline-binding domain. The Src SH2 domain binds to phospho-Tyr527 (phosphorylated by Csk) within the Src molecule, causing Src to adopt an inactive conformation. Mutation of Tyr527 to phenylalanine removes the SH2-binding target and results in a constitutively active kinase. A second mutation at the conserved lysine residue at the ATP-binding site abolishes kinase activity, but without Tyr527, the conformation of the protein is open, allowing interaction with endogenous activators. Consequently, this double-mutant exhibits a dominant-negative phenotype

~ 60 kDa

Immunogen

N-terminal 6His-tagged recombinant full-length human Src expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Application

Research Category
Cell Structure

Research Sub Category
Cytoskeletal Signaling

This Anti-Src Antibody, clone N6L is validated for use in WB for the detection of Src.

Biochem/physiol Actions

Recognizes Src. Crossreacts with Yes and weakly with Fyn. No crossreactivity observed with Lck or Lyn.

Physical form

Cultured supernantant containing 0.05% sodium azide.

Preparation Note

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Included positive antigen control of non-stimulated A431 lysate (Cat. No. 12-301). Add 2.5 μL of 2-mercaptoethanol/100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

Western Blot Analysis: A 1:500 to 1:1000 dilution of this antibody detected Src in RIPA lysates from A431 cells.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 05-889

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• Lagerklasse: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable