03-0171-00

SMC^® Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit

Used for ultrasensitive quantification of Human BDNF in human serum, plasma, and cerebrospinal fluid (CSF)

• Synonym(e): Abrineurin immunoassay kit, BDNF Single Molecule Counting Kit, BDNF ultra-sensitive immunoassay
• NACRES: NA.84
• UNSPSC Code: 12161503
• Technique(s): single molecule counting: suitable
• Usage: kit sufficient for 96 wells
• Compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments

Eigenschaften

• Produktname: SMC^® Human Brain-Derived Neurotrophic Factor (BDNF) High Sensitivity Immunoassay Kit,
• description: Used for ultrasensitive quantification of Human BDNF in human serum, plasma, and cerebrospinal fluid (CSF)
• product line: SMC^®
• usage: kit sufficient for 96 wells
• technique(s): single molecule counting: suitable
• input: serum; cerebrospinal fluid (CSF); plasma (K2 EDTA)
• UniProt accession no.: (P22360)
• compatibility: for use with FemtoQuest^™ and SMCxPRO^® instruments
• relevant disease(s): Alzheimer′s disease
• shipped in: wet ice
• storage temp.: 2-8°C

Beschreibung

General description

Brain-Derived Neurotrophic Factor (BDNF) is a crucial protein involved in the development, maintenance, and survival of neurons in the brain. As a member of the neurotrophin family, BDNF promotes neuronal growth, differentiation, and synaptic plasticity, which are essential for learning and memory. It is primarily expressed in the brain, particularly in regions such as the hippocampus, cortex, and basal forebrain.

BDNF plays a significant role in neurological disorders and neurodegenerative diseases. Reduced levels of BDNF have been associated with conditions like Alzheimer′s disease, Parkinson′s disease, and depression. Low BDNF levels can lead to neuronal atrophy, impaired synaptic function, and increased vulnerability to neuroinflammation, exacerbating disease progression. Conversely, enhancing BDNF signaling has been shown to promote neuroprotection, improve cognitive function, and support recovery in animal models of neurodegeneration.

Understanding BDNF′s function and its involvement in neuroinflammation and neurodegenerative processes is crucial for developing therapeutic strategies aimed at enhancing BDNF signaling to improve outcomes for neurological disorders.

Features and Benefits

• Ultra-Sensitive Measurement: Accurately measures BDNF at femtogram/mL concentrations in serum, plasma, or CSF.
• Enhanced Signal Clarity: Improves the signal-to-noise ratio, minimizing background noise for consistent assay results.
• Intuitive Workflow: Employs a familiar sandwich immunoassay design, facilitating easy adoption for researchers trained in ELISA.
• Ready for Automation: Designed to integrate with automated liquid handling systems, streamlining processes and boosting laboratory productivity.
• Comprehensive Support: Supported by experienced field scientists and technical experts.

Packaging

96 well assay

Analysis Note

• Sensitivity: LLOQ 40 fg/mL* (SMCxPRO^® Instrument)
• Standard Curve Range: 20-100,000 fg/mL
• Intra-assay CV: <10%
• Inter-assay CV: <15%
• Linearity: Serum: 85% Plasma: 102%
• Recovery: Serum: 90% Plasma: 96% CSF: 98%

*Indicative LLOQ created from average of 14 independent in house runs, where >80% met same value defined with CV & Recovery +/- 20%. Refer to CoA for lot specific performance.

NeuroscienceNeurological DisordersNeurotropins

Other Notes

Although BDNF was detectable in mouse, rat, non-human primate (rhesus macaque and cynomolgus monkey), and canine samples, the assay has not been verified for these species.

Legal Information

FEMTOQUEST is a trademark of Merck KGaA, Darmstadt, Germany

SMC is a registered trademark of Singulex

SMCxPRO is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Sicherheitshinweise

• pictograms: GHS06,GHS08
• signalword: Danger
• hcodes: H302 + H332,H311,H319,H360FD,H373,H412,EUH430
• pcodes: P201 - P202 - P273 - P280 - P308 + P313 - P391 - P405 - P501
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - ED ENV 1 - Eye Irrit. 2 - Repr. 1B - STOT RE 2 Inhalation
• target_organs: Respiratory Tract
• Lagerklasse: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3