U7128

Uricase from Arthrobacter globiformis

lyophilized powder, 15-30 units/mg protein (biuret)

• Synonym(e): Urate oxidase, Urate:O₂ oxidoreductase
• CAS-Nummer: 9002-12-4
• EC-Nummer: 1.7.3.3 (BRENDA, IUBMB)
• EG-Nummer: 232-655-5
• MDL-Nummer: MFCD00082126
• UNSPSC-Code: 12352204
• PubChem Substanz-ID: 24900663
• NACRES: NA.54

Eigenschaften

• Form: lyophilized powder
• Spezifische Aktivität: 15-30 units/mg protein (biuret)
• Mol-Gew.: tetramer ~32 kDa
• Lagertemp.: −20°C
• SMILES String: [P](=O)([O-])(OC3C(OC(C3O)N4C=CC(=O)NC4=O)CO)OCC1OC(C(C1O)O)N2C=CC(=O)NC2=O.[N+H4]
• InChI: 1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
• InChIKey: VPXDHZMLJOJKOX-UHFFFAOYSA-N

Beschreibung

Allgemeine Beschreibung

Research area: Cell Signalling. Uricase is made of four identical subunits and is a homotetrameric enzyme. It is found as a functional uricase in several organisms including, bacteria, archaea, and eukaryotes. Nonetheless, primates and humans lack a functional uricase enzyme.[1]

Anwendung

Uricase from Arthrobacter globiformis has been used for coating the electrodes in - biosensor fabrication for the detection of uric acid in urine samples.[2] It has also been used for analysing the effect of Zn, Cu, Mg, and Mn ions on uricase.[3]

Biochem./physiol. Wirkung

Arthrobacter globiformis is a major producer of uricase or urate oxidase in poultry manure and litter. Uricase hydrolyzes uric acid and causes ammonia volatilization in poultry manure.[3] In the majority of mammals, uricase converts uric acid to allantoin, a soluble excretion product. Humans lack uricase due to its loss during the evolution of hominids.[4] This increases the risk of humans towards gout, kidney stones, hypertension, and diabetes due to increased circulating levels of uric acid.[5]Uricase is an important enzyme that plays a role in the purine metabolic pathway.[6]

Molecular weight: 4 × ~32 KDa (tetramer)
Isoelectric point: is 4.64
K_M value (uric acid as substrate): ~ 0.01 mM
The optimum pH for activity: 8.5-9.5
The optimum pH for stability: 6.0-9.5

Zn and Cu were shown to inhibit the activity of uricase. [7]

Einheitendefinition

One unit will convert 1.0 μmole of uric acid to allantoin per min at pH 9.0 at 25 °C.

Sicherheitshinweise

• Piktogramme: GHS08
• Signalwort: Danger
• H-Sätze: H334
• P-Sätze: P261 - P284 - P501
• Gefahreneinstufungen: Resp. Sens. 1
• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)