U0880

Uricase from Candida sp.

recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid

• Synonym(e): Urate: oxygen oxoreductase
• CAS-Nummer: 9002-12-4
• UNSPSC Code: 12352204
• PubChem Substance ID: 24900619
• NACRES: NA.54
• EG-Nummer: 1.7.3.3 (BRENDA, IUBMB)
• MDL number: MFCD00082126
• Specific activity: ≥2 units/mg solid
• Recombinant: expressed in E. coli

Eigenschaften

• recombinant: expressed in E. coli
• Quality Segment: 200
• form: lyophilized powder
• specific activity: ≥2 units/mg solid
• mol wt: 35 kDa by SDS-PAGE
• storage temp.: −20°C
• SMILES string: [P](=O)([O-])(OC3C(OC(C3O)N4C=CC(=O)NC4=O)CO)OCC1OC(C(C1O)O)N2C=CC(=O)NC2=O.[N+H4]
• InChI: 1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
• InChI key: VPXDHZMLJOJKOX-UHFFFAOYSA-N

Beschreibung

General description

Uricase deficient mice develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment.

Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.

Application

Uricase from Candida sp. has been used:

• to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
• to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
• for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors

Biochem/physiol Actions

Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.

Physical form

Powder; contains citrate and sucrose

Other Notes

One unit will oxidize 1.0 μmole of uric acid to allantoin per minute at pH 8.5 at 25 °C.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable