T7705

Thimet Oligopeptidase from Bacillus licheniformis

• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.4.24.15 (BRENDA, IUBMB)
• Form: powder
• Storage temp.: −20°C

Eigenschaften

• recombinant: expressed in E. coli
• Quality Segment: 200
• form: powder
• mol wt: ~77 kDa
• storage temp.: −20°C

Beschreibung

General description

Thimet oligopeptidase is considered essential in the degradation of collagen in collaboration with collagenolytic enzymes. Thimet oligopeptidase (TOP) is a neuropeptidase involved in the hydrolysis of gonadotropin-releasing hormone, a key component of the hypothalamic-pituitary-gonadal axis. [1]

Application

Thimet oligopeptidase can be used for the degradation of collagen in combination with collagenases. It can also be used for the hydrolysis of neuropeptides such as bradykin, neurotensin, and amyliod-β-peptide. Thimet oligopeptidase has been used in a study to investigate the effect of acute cocaine administration in male rats on TOP specific activity and mRNA levels in prosencephalic brain areas related with the reward circuitry: ventral striatum, hippocampus, and frontal cortex. [2]

Preparation Note

This enzyme has been affinity chromatographically purified using a niquel affinity column. It contains a 6-Histidine tag in its C-terminus.

A working solution of this enzyme can be prepared in 20 mM phosphate buffered saline solution, pH 7.0, or sterile and deionized water, pH 7.0.

Sicherheitshinweise

• pictograms: GHS07
• signalword: Warning
• hcodes: H315,H332
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P304 + P340 + P312
• Hazard Classifications: Acute Tox. 4 Inhalation - Skin Irrit. 2
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable