T0319

Trizma^® Tris-Kristalle

BioPerformance Certified, pH 7.4, average M_w 151.6

• Synonym(e): Tris-Kristalle
• NACRES: NA.25
• UNSPSC Code: 12161700

Eigenschaften

• grade: BioPerformance Certified
• Quality Segment: 200
• form: crystalline powder
• mol wt: average M_w 151.6
• technique(s): cell culture | mammalian: suitable
• impurities: DNase, RNase, Protease, NICKase, none detected, endotoxin, tested, total aerobic microbial count, tested, ≤0.05 A₂₉₀, ≤0.5% water (Karl Fischer), ≤5 ppm Heavy metals (as Pb)
• pH: 7.12 (37 °C, 0.1 M)(lit.), 7.40 (25 °C, 0.1 M)(lit.), 7.4, 7.97 (5 °C, 0.1 M)(lit.)
• useful pH range: 7.0-9.0
• solubility: deionized water: soluble at 25 °C, clear, colorless (pH 7.4, 1 pouch dissolved in 1 L to yield a 0.1 M Trizma solution)
• application(s): diagnostic assay manufacturing

Beschreibung

Application

Das Produkt wird in Zellkultur getestet. Es wird außerdem auf niedrige Endotoxinwerte getestet und auf die Abwesenheit von Nukleasen und Proteasen analysiert.

Open the foil pouch, dissolve contents in 900 mL of water; add additional water to a final volume of 1 liter to make a 0.1 M solution at a temperature of 25 °C. No pH adjustment is necessary. Guaranteed accuracy: ±0.1 pH units.

Tris has a significant temperature coefficient, so select the mixture to give the desired final pH at the desired temperature.
From 5 °C to 25 °C, the pH decreases an average of 0.03 pH units per °C.
From 25 °C to 37 °C, the pH decreases an average of 0.025 pH units per °C.

If a different concentration is desired, note that there is a concentration effect on the pH of the solution.

Packaging

In convenient foil pouches

Preparation Note

Trizma-Lösungen können autoklaviert werden.

Other Notes

Bestimmte pH-Elektroden liefern mit Tris-Puffern keine genauen Ergebnisse.

This product is a pre-mixed combination of Trizma base and Trizma HCl in convenient foil pouches that can be used to prepare a common pH value for Tris buffer.

Legal Information

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves