P7643

PIPES Dikaliumsalz

≥99% (titration)

• Synonym(e): 1,4-Piperazindiethansulfonsäure Dikaliumsalz, Piperazin-1,4-bis-(2-ethansulfonsäure) Dikaliumsalz
• Empirische Formel (Hill-System): C₈H₁₆K₂N₂O₆S₂
• CAS-Nummer: 108321-27-3
• Molekulargewicht: 378.55
• MDL-Nummer: MFCD00069751
• UNSPSC-Code: 12161700
• PubChem Substanz-ID: 24898738
• NACRES: NA.25

Eigenschaften

• Assay: ≥99% (titration)
• Form: crystalline powder
• Nützlicher pH-Bereich: 6.1-7.5
• pKa (25 °C): 6.8
• Löslichkeit: water: 0.25 g/mL, clear, colorless
• Anwendung(en): diagnostic assay manufacturing
• SMILES String: [K+].[K+].[O-]S(=O)(=O)CCN1CCN(CC1)CCS([O-])(=O)=O
• InChI: 1S/C8H18N2O6S2.2K/c11-17(12,13)7-5-9-1-2-10(4-3-9)6-8-18(14,15)16;;/h1-8H2,(H,11,12,13)(H,14,15,16);;/q;2*+1/p-2
• InChIKey: BJQYFCAMUXGZFN-UHFFFAOYSA-L

Beschreibung

Allgemeine Beschreibung

PIPES (1,4-Piperazinediethanesulfonic acid) is a zwitterionic buffer. It is a stable buffer and is used majorly in fixation solutions. PIPES is inert towards enzymatic and non-enzymatic reactions. However, it generates artefacts in transmission electron microscope imaging.

Anwendung

PIPES (1,4-Piperazinediethanesulfonic acid) dipotassium salt has been used as buffer for fluorescence polarization microscopy studies of kinesin-microtubule binding. It is suitable for use as a component of PHEM buffer ([K-PIPES], HEPES, EGTA, and MgSO4) used during rinsing and blocking steps in immunofluorescence imaging of HeLa cells. It is also suitable for use as buffer for purification of tubulin.

Sicherheitshinweise

• Lagerklassenschlüssel: 11 - Combustible Solids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable
• Persönliche Schutzausrüstung: Eyeshields, Gloves, type N95 (US)