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P4474

Phosphatgepufferte Kochsalzlösung

liquid, pH 7.4, sterile-filtered, suitable for cell culture

Synonym(e):

Phosphatgepufferte Kochsalzlösung -Lösung, PBS

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PackungsgrößeSKUVerfügbarkeitPreis
500 mL
Warenkorb auf Verfügbarkeit prüfen
€ 22,10
1 L
Warenkorb auf Verfügbarkeit prüfen
€ 35,70

Über diesen Artikel

UNSPSC Code:
12352207
NACRES:
NA.25
MDL number:

€ 22,10


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Produktname

Phosphatgepufferte Kochsalzlösung, pH 7.4, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

Quality Segment

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

pH

7.4

General description

Phosphate-buffered saline (PBS) is a buffer solution used in biological research. It is a water-based salt solution containing sodium phosphate, sodium chloride and, in some formulations, it contains potassium chloride and potassium phosphate. The osmolality and ion concentrations of the solutions match those of the human body (isotonic) and are non-toxic to most cells.

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Dieser Artikel
P2272P5119P4417
technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

form

liquid

form

liquid

form

liquid

form

tablet

pH

7.4

pH

7.2

pH

7.2 (25 °C)

pH

7.2-7.6 (1 tablet/200 mL), 7.4

sterility

sterile-filtered

sterility

sterile-filtered

sterility

autoclaved

sterility

-

impurities

Endotoxin, tested

impurities

Endotoxin, tested

impurities

-

impurities

-


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Lagerklasse

10 - Combustible liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–6 of 6 Questions  
  1. Is this PBS Ca²⁺/Mg²⁺-free? 

    1 answer
    1. This product does not contain calcium or magnesium. The concentration of each component is as follows:
      - 0.144 g/L / 1.058mM Potassium phosphate monobasic
      - 9.0 g/L / 154mM Sodium chloride
      - 0.528 g/L / 2.97mM Sodium phosphate dibasic dihydrate

      Helpful?

  2. Why does this PBS not contain potassium chloride?

    1 answer
    1. Phosphate-buffered saline is a salt solution containing sodium phosphate and sodium chloride. In some formulations, it also contains potassium chloride and potassium phosphate. For example, products P4417 and P2272 contain both potassium chloride and sodium chloride.

      Helpful?

  3. Is it DNAs free?

    1 answer
    1. This product is intended for cell culture use and is not assayed for the presence of endonucleases. The specifications can be found at: https://www.sigmaaldrich.com/specification-sheet/SIGMA/P4474

      Helpful?

  4. What is the conductivity of this solution?

    1 answer
    1. Unfortunately, the conductivity is not tested for this product as this is not a specification.

      Helpful?

  5. What is the concentration (in mM) of this solution?

    1 answer
    1. This product is a multicomponent item. The concentration and molarity of each component is as follows:
      Potassium phosphate monobasic - 0.144 g/L or 1.058mM
      Sodium chloride - 9.0 g/L or 154mM
      Sodium phosphate dibasic dihydrate - 0.528 g/L or 2.97mM

      Helpful?

  6. What is the composition of P4474 Phosphate buffered saline pH 7.4, liquid, sterile-filtered, suitable for cell culture ?

    1 answer
    1. Product P4474 contains the following:
      - 0.144 g/L Potassium phosphate monobasic
      - 9.0 g/L Sodium chloride
      - 0.528 g/L Sodium phosphate dibasic dihydrate

      Helpful?

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