M7383

Äpfelsäure-Dehydrogenase aus Schweineherz

≥400 units/mg protein (biuret), ammonium sulfate suspension

• Synonym(e): L-Malate: NAD⁺ oxidoreductase, MDH
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-622-5
• MDL number: MFCD00131567
• EG-Nummer: 1.1.1.37 (BRENDA, IUBMB)
• Specific activity: ≥400 units/mg protein (biuret)

Eigenschaften

• form: ammonium sulfate suspension
• specific activity: ≥400 units/mg protein (biuret)
• foreign activity: Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%
• storage temp.: 2-8°C

Beschreibung

General description

Cytoplasmic Isozyme

Application

Malic dehydrogenase has been used in a study to assess the use of a long-wavelength homogeneous enzyme immunoassay for the determination of amikacin in water samples. [1] It has also been used in a study to investigate the lipid metabolism in a liver exposed to cadmium. [2]

Biochem/physiol Actions

The surfactant cetyl (hexadecyl) trimethylammonium bromide inhibits the cytoplasmic isoform of porcine heart malate dehydrogenase but has no effect on the mitochondrial form. SDS however; inhibits both isoforms. [3]

Physical form

Suspension in 3.2 M (NH₄)₂SO₄, 0.1 M KH₂PO₄, pH 7.0

Other Notes

One unit will convert 1.0 μmole of oxalacetate and β-NADH to _L-malate and β-NAD per min at pH 7.5 at 25 °C.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)