M1567

Äpfelsäure-Dehydrogenase aus Schweineherz

≥600 units/mg protein (biuret), ammonium sulfate suspension

• Synonym(e): L-Malate: NAD⁺ oxidoreductase, MDH
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-622-5
• MDL number: MFCD00131567
• EG-Nummer: 1.1.1.37 (BRENDA, IUBMB)
• Specific activity: ≥600 units/mg protein (biuret)

Eigenschaften

• form: ammonium sulfate suspension
• Quality Segment: 200
• specific activity: ≥600 units/mg protein (biuret)
• foreign activity: Glutamic-Oxalacetic Transaminase ≤0.01%, Glutamic-Pyruvic Transaminase ≤0.01%
• storage temp.: 2-8°C

Beschreibung

General description

Malic dehydrogenase exists as a dimer with each subunit containing an NAD-binding domain and a substrate-binding carboxy-terminal domain required for activity.

Malic dehydrogenase is a cytoplasmic isozyme and an important catalyst in the tricarboxylic acid cycle.

Malic Dehydrogenase is a ubiquitous enzyme, which exists in two isoforms in eukaryotic cells.

Application

Malic dehydrogenase has been used in a study to assess the effect of an immunomodulator S2 complex on the enzymes of the parasites. It has also been used in a study to investigate the heterogeneity of lactic and malic dehydrogenase in cerebrospinal fluid.

Malic Dehydrogenase from porcine heart has been used:

• in qualitative protein binding measurements
• to test internally calibrated electrochemical continuous enzyme assay (ICECEA) with model enzyme pair
• to investigate the effect of chaperone on the refolding of heat-denatured malate dehydrogenase

Biochem/physiol Actions

Malic Dehydrogenase (MDH) plays an important role in the citric acid cycle in mitochondria. It catalyzes the interconversion of substrates malate and oxaloacetate with the simultaneous oxidation/reduction of NAD/NADH⁺. MDH present in the cytosol is involved in the shuttling of malate/aspartate.

Physical form

Suspension in 2.8 M (NH₄)₂SO₄ -Lösung, pH 6.0

Other Notes

1 U setzt 1.0 μmol Oxalacetat und β-NADH zu L-Aepfelsäure-ester und β-NAD pro Minute um bei pH 7.5 und 25°C.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)