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M0269

Methotrexat−Agarose

saline suspension

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PackungsgrößeSKUVerfügbarkeitPreis
10 mL
Warenkorb auf Verfügbarkeit prüfen
€ 249,00

Über diesen Artikel

UNSPSC Code:
13111023
NACRES:
NA.56
MDL number:
MFCD00164466

€ 249,00

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Technischer Dienst
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Unterstützung erhalten

biological source

plant

Quality Level

200

form

saline suspension

extent of labeling

2-7 mg per mL

technique(s)

affinity chromatography: suitable

matrix

cross-linked 4% beaded agarose

matrix activation

cyanogen bromide

matrix attachment

carboxyl

matrix spacer

8 atoms

suitability

suitable for chromatography

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Dieser Artikel
H0402M9929PHR1396
Methotrexate−Agarose saline suspension

Sigma-Aldrich

M0269

Methotrexate−Agarose

Heparin−Agarose (1:1 suspension in a 20% ethanol solution)

Sigma-Aldrich

H0402

Heparin−Agarose

Methotrexate meets USP testing specifications

Sigma-Aldrich

M9929

Methotrexate

Methotrexate Pharmaceutical Secondary Standard; Certified Reference Material

Supelco

PHR1396

Methotrexate

form

saline suspension

form

(1:1 suspension in a 20% ethanol solution)

form

powder

form

-

Quality Level

200

Quality Level

200

Quality Level

400

Quality Level

300

biological source

plant

biological source

heparin from Porcine intestinal mucosa

biological source

synthetic

biological source

-

suitability

suitable for chromatography

suitability

-

suitability

-

suitability

-

technique(s)

affinity chromatography: suitable

technique(s)

-

technique(s)

activity assay: suitable

technique(s)

HPLC: suitable, gas chromatography (GC): suitable

extent of labeling

2-7 mg per mL

extent of labeling

-

extent of labeling

-

extent of labeling

-

Application

Methotrexate-agarose is used in protein chromatography, affinity chromatography, metabolic pathways and specialty resins. Methotrexate-agarose has been used to investigate the toxicity mechanism of mortality in adult buffalo flies caused by ingestion of folate analogues. Methotrexate-agarose has also been used to study the purification, cloning, and functional expression of dihydroneopterin triphosphate 2′-epimerase from Escherichia coli.

Physical form

Suspension in 1.0 M NaCl, enthält 0.02% Thiomersal

Lagerklasse

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000362898
0000313030
0000313030
0000362898
0000341705

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S Rospert et al.
The EMBO journal, 15(4), 764-774 (1996-02-15)
Proteins that are imported from the cytosol into mitochondria cross the mitochondrial membranes in an unfolded conformation and then fold in the matrix. Some of these proteins require the chaperonin hsp60 for folding. To test whether hsp60 is required for
C J Thomson et al.
Journal of general microbiology, 136(4), 673-677 (1990-04-01)
The type IIIb dihydrofolate reductase, a novel plasmid-encoded enzyme recently identified in Shigella sonnei, has been shown to have some similar biochemical properties to the type IIIa dihydrofolate reductase which was first identified in New Zealand in 1979. However, the
V Wilquet et al.
European journal of biochemistry, 255(3), 628-637 (1998-09-17)
We have overexpressed the gene for dihydrofolate reductase (DHFR) from Thermotoga maritima in Escherichia coli and characterized the biochemical properties of the recombinant protein. This enzyme is involved in the de novo synthesis of deoxythymidine 5'-phosphate and is critical for
P Loetscher et al.
The Journal of biological chemistry, 266(17), 11213-11220 (1991-06-15)
Several years ago, we proposed that polypeptide regions rich in proline (P), glutamic acid (E), serine (S), and threonine (T) (PEST) target intracellular proteins for destruction (Rogers, S., Wells, R., and Rechsteiner, M. (1986) Science 234, 364-368). To test the
K Braig et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(9), 3978-3982 (1993-05-01)
Chaperonins are oligomeric protein complexes that play an essential role in the cell, mediating ATP-dependent polypeptide chain folding in a variety of cellular compartments. They appear to bind early folding intermediates, preventing their aggregation; in the presence of MgATP and
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Global Trade Item Number

SKUGTIN
M0269-10ML04061833992913
M0269-25ML04061833992920

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