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Merck

L2158

L-Leucine p-nitroanilide hydrochloride

chromogenic, ≥98% (HPLC), powder

Synonym(e):

H-Leu-pNA

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Über diesen Artikel

Empirische Formel (Hill-System):
C12H17N3O3 · HCl
CAS-Nummer:
Molekulargewicht:
287.74
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.28
MDL number:

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Produktname

L-Leucine p-nitroanilide hydrochloride, ≥98% (HPLC)

SMILES string

Cl.CC(C)C[C@H](N)C(=O)Nc1ccc(cc1)N(=O)=O

InChI

1S/C12H17N3O3.ClH/c1-8(2)7-11(13)12(16)14-9-3-5-10(6-4-9)15(17)18;/h3-6,8,11H,7,13H2,1-2H3,(H,14,16);1H/t11-;/m0./s1

InChI key

PFOYXOWPGRWPLS-MERQFXBCSA-N

assay

≥98% (HPLC)

form

powder

solubility

water: 25 mg/mL

storage temp.

2-8°C

Quality Level

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Dieser Artikel
V0882A9325A2559
assay

≥98% (HPLC)

assay

≥98% (TLC)

assay

≥99% (TLC)

assay

≥97% (HPLC)

solubility

water: 25 mg/mL

solubility

water: 50 mg/mL, clear, colorless to yellow

solubility

water: 50 mg/mL, clear to very slightly hazy

solubility

-

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

100

form

powder

form

powder

form

powder

form

powder

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

Substrate for the colorimetric determination of leucine aminopeptidase.

Application

L-Leucine p-nitroanilide hydrochloride has been used as a substrate to measure the activity of leucine aminopeptidase (LAP):
  • for spectrophotometric assays
  • in the intestinal samples, such as duodenum, jejunum, and ileum[1]
  • in the hemolymph of Manila clam[2]

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Lagerklasse

11 - Combustible Solids


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Susan E Ford et al.
Fish & shellfish immunology, 23(2), 280-291 (2006-12-19)
Components of the haemolymph are understood to constitute the internal defense system of bivalve mollusks and their levels are often considered to be indicators of "health"; however, relatively little proof exists of the role that these elements play in the
Arun Kumar Singh et al.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 16(5), e375-e381 (2012-03-14)
Staphylococcus aureus has emerged as a major drug-resistant pathogen in hospital- and community-acquired infections. Leucine aminopeptidase (LAP) is known to be essential for survival of the bacteria; however the LAP of S. aureus has not been extensively characterized. In this
J Williams et al.
British poultry science, 49(3), 329-339 (2008-06-24)
1. The objective of this experiment was to study two feeding methods, which could potentially act on the gut microflora, the structure and/or the function of the digestive tract and thereby improve the performance of broilers. 2. Four dietary treatments
Characterization of proteolytic enzyme activities in macroalgae
Perez-Llorens JL, et al.
European Journal of Phycology, 38(1), 55-64 (2003)
Arun Kumar Singh et al.
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases, 16(5), e375-e381 (2012-03-14)
Staphylococcus aureus has emerged as a major drug-resistant pathogen in hospital- and community-acquired infections. Leucine aminopeptidase (LAP) is known to be essential for survival of the bacteria; however the LAP of S. aureus has not been extensively characterized. In this

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