This product is sold sterile.
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L1668
Lektin aus Phaseolus vulgaris (rote Kidneybohne)
Phytohemagglutinin PHA-P, lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl), BioReagent, suitable for cell culture
Synonym(e):
Phaseolus vulgaris Agglutinin, PHA
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| Ihnen/SKU | Verfügbarkeit | Preis |
|---|---|---|
5 mg | Warenkorb auf Verfügbarkeit prüfen | € 87,00 |
Über diesen Artikel
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)
€ 87,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenQuality Segment
product line
BioReagent
form
lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl)
potency
≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)
technique(s)
cell culture | mammalian: suitable
color
white to off-white
solubility
PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)
storage temp.
2-8°C
Application
Lektin aus Phaseolus vulgaris (Gartenbohnen) hat folgende Anwendungen:
- Stimulation von Differenzierungscluster (CD4+)-T-Zellen
- Aktivierung von primären Lymphozyten des peripheren Bluts
- Stimulation von mononuklearen Zellen des peripheren Bluts
Biochem/physiol Actions
PHA besteht aus zwei Molekularspezies, einem Erythroagglutinin (PHA-E) mit niedriger mitogener und hoher erythroagglutinierender Aktivität sowie einem Leucoagglutinin (PHA-L) mit hoher mitogener und hoher leucoagglutinierender Aktivität, aber sehr niedriger erythroagglutinierender Aktivität.
PHA-E ist nicht blutgruppenspezifisch, die Agglutination kann aber durch bestimmte Oligosaccharide gehemmt werden. PHA-P ist die Proteinform von PHA vor Trennung und Aufreinigung von Erythroagglutinin und Leucoagglutinin. PHA-M ist die Mucoproteinform. Konjugate werden aus den entsprechenden aufgereinigten Lektinen zubereitet.
PHA-E ist nicht blutgruppenspezifisch, die Agglutination kann aber durch bestimmte Oligosaccharide gehemmt werden. PHA-P ist die Proteinform von PHA vor Trennung und Aufreinigung von Erythroagglutinin und Leucoagglutinin. PHA-M ist die Mucoproteinform. Konjugate werden aus den entsprechenden aufgereinigten Lektinen zubereitet.
Packaging
Vertrieb auf Basis des Lektingehalts
Analysis Note
Stärke und Löslichkeit ausgedrückt in mg Lektin/ml
1 of 1
Dieser Artikel | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) - |
| form lyophilized powder (contains ~50% buffer salts as sodium phosphate and NaCl) | form lyophilized powder | form lyophilized powder | form lyophilized powder |
| solubility PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8) | solubility PBS: 2 mg/mL | solubility DPBS: 5 mg/mL, slightly hazy, colorless | solubility - |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| potency ≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells) | potency <40 μg per mL agglutination activity | potency erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.), ≤1.0 μg per mL leucoagglutination activity, ≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells) | potency erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.), ≤1.0 μg/mL leucoagglutination activity, ≤5 μg/mL mitogenic activity |
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Lagerklasse
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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In the specification sheet, it is stated that the product is sterile. In all other documents or descriptions, it is not mentioned if this product is sterile or not. Could it be confirmed that this product is always sterile?
1 answer-
Helpful?
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What do the designations -P, -L, -M, and -E represent in the names of PHA products?
1 answer-
Generally, PHA-P or PHA-L is utilized to stimulate lymphocytes, although PHA-M will also work, but not to the same degree. The most active form is PHA-L (the mitogenic concentration is < 5μg/ml). PHA, or Phytohemagglutinin, comprises two molecular species:
1. Erythroagglutinin (PHA-E), which demonstrates low mitogenic activity and high erythroagglutinating activity.
2. Leucoagglutinin (PHA-L), which possesses high mitogenic and leucoagglutinating activity, accompanied by very low erythroagglutinating activity.
PHA-P represents the protein form of PHA before the erythroagglutinin and leucoagglutinin components are separated and purified. PHA-M refers to the mucoprotein form of Phytohemagglutinin.
Helpful?
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How should the PHA solution using Phytohemagglutinin PHA-P powder be prepared for cell culture?
1 answer-
The stock solution of this product can be prepared in phosphate buffered saline at pH 6.8, resulting in a clear to slightly hazy solution at a concentration of 5 mg per mL. This stock solution can then be diluted in cell culture medium to a working concentration of 10 micrograms of lectin per mL for use in peripheral blood cell culture.
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What is the shelf life once reconstituted in PBS?
1 answer-
After reconstitution in PBS, this product can be stored at 2-8°C for 2-4 weeks. PHA loses approximately 30% activity after 1 month. Stock solutions of at least 1 mg/mL can be stored in frozen aliquots. Do not dilute to the working concentration until ready to use. Vortex stock solutions prior to use.
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What is the mitogenic activity of Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder?
1 answer-
The minimum specification for mitogenic activity of this product is < or = 10 micrograms lectin/mL of medium. The reported specification is PASS. A specific value is not given.
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What is the solution stability for Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder?
1 answer-
The solution stability was not evaluated by Sigma-Aldrich; however, references indicate media containing dilute PHA can be stored refrigerated for 2-4 weeks. PHA loses approximately 30% activity after 1 month. Stock solutions can be stored in frozen aliquots.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How can Product L1668, Lectin from Phaseolus vulgaris (red kidney bean) - Phytohemagglutinin PHA-P, lyophilized powder, be dissolved?
1 answer-
Product No. L1668 is solubility tested using 5 mg per mL of phosphate buffered saline (PBS), pH 6.8; this yields a clear to slightly hazy, colorless to light yellow solution.
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