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H7379

Hämoglobin human

Name: Hämoglobin human
Brand: SIGMA
Price: 87.3 EUR

lyophilized powder

Synonym(e):

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Packungsgröße	Verfügbarkeit	Preis
1 g	Warenkorb auf Verfügbarkeit prüfen	€ 87,30
5 g	Warenkorb auf Verfügbarkeit prüfen	€ 258,00
10 g	Warenkorb auf Verfügbarkeit prüfen	€ 461,00

Über diesen Artikel

CAS-Nummer:

9008-02-0

UNSPSC Code:

12352202

eCl@ss:

42030116

NACRES:

NA.61

MDL number:

MFCD00131282

Form:

lyophilized powder

Biological source:

human

€ 87,30

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Technischer Dienst

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Unterstützung erhalten

Eigenschaften

biological source

human

Quality Segment

200

form

lyophilized powder

technique(s)

enzyme immunoassay: suitable

impurities

HIV and hepatitis B antigen, tested negative

solubility

H₂O: soluble 20 mg/mL

UniProt accession no.

P02008, P02042, P02100, P09105, P68871, P69891, P69892, P69905

storage temp.

2-8°C

Gene Information

human ... HBA1(3039), HBA2(3040), HBB(3043), HBD(3045), HBE1(3046), HBG1(3047), HBG2(3048), HBQ1(3049), HBZ(3050)

Beschreibung

General description

Hämoglobin ist die Hauptkomponente von Erythrozyten und für deren rote Farbe verantwortlich. Seine normale Konzentration in den Erythrozyten ist 34 %. Hämoglobin ist aufgrund seiner Fähigkeit zum Transport des Sauerstoffes von der Lunge zu den Organgeweben und Austausch des Kohlenstoffdioxids, das wichtigste respiratorische Protein von Wirbeltieren.

Application

Hämoglobin wurde in der Entwicklung eines schnell durchführbaren Enzym-Immunoassays für die Bestimmung von retinolbindendem Protein verwendet.[1] Es wurde auch für die Hemmung der Reaktivität von humanen Thrombozyten durch den endothelialen relaxierenden Faktor verwendet.[2]

Biochem/physiol Actions

Das Gleichgewicht zwischen Fe²⁺ und Fe³⁺ ist ein physiologischer Indikator für die Oxygenierung des Blutes. Desoxygeniertes Hämoglobin liefert einen Rückführungsmechanismus, indem Nitrit zu NO reduziert wird, d. h. zu einem Vasodilatator, der den Blutfluss an sauerstoffarmes Gewebe erhöht.

Sauerstofftransporter, NO-Fänger

Disclaimer

Da natives Hämoglobin an der Luft spontan oxidiert, können diese Zubereitungen vorwiegend Methämoglobin enthalten.

NUR FÜR FORSCHUNGSZWECKE. Dieses Produkt unterliegt in Frankreich den Vorschriften für den Gebrauch zu wissenschaftlichen Zwecken, auch bei der Ein- und Ausfuhr (Artikel L 1211-1 Absatz 2 des französischen Gesundheitsgesetzes). Der Käufer (d. h. der Endverbraucher) ist verpflichtet, eine Einfuhrgenehmigung des französischen Forschungsministeriums gemäß Artikel L1245-5-1 II. des französischen Gesundheitsgesetzes einzuholen. Mit der Bestellung dieses Produkts bestätigen Sie, dass Sie über die entsprechende Einfuhrgenehmigung verfügen.

Questions

1–10 of 20 Questions

a year ago

Can I use this product to validate hemolysis testing on chemistry analyzer.

1 answer
1. Technical Support
  
  7 months ago
  
  This product is a research use only (RUO) item and is not specifically recommended for validating hemolysis testing on a chemistry analyzer.
  
  Helpful?
10 months ago

Would H7379 Hemoglobin Human has population of glycated hb as well? If yes, what level?

1 answer
1. Technical Support
  
  10 months ago
  
  MilliporeSigma, on product H7379, does not perform any sort of characterization as to whether any particular subfraction of H7379 is glycated. H7379 is a general hemoglobin product and not any single one isoform.
  
  Helpful?
Anonymous

a year ago

I would like to know if the product loses stability if it is kept in a freezer at -20, since the recommended temperature is between 2 and 8 degrees Celsius.

1 answer
1. Technical Support
  
  11 months ago
  
  The stability of the hemoglobin powder at -20°C has not been evaluated. However, storage of the powder at freezer temperature is not expected to cause degradation. Please see the link below to review additional information available in the product datasheet:
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/837/353/h7379pis.pdf
  
  Helpful?
a year ago

What are the recommendations for long-term storage once it is solubilized? Is it OK to make single-use aliquots for -80°C?

1 answer
1. Technical Support
  
  a year ago
  
  The stability of this material in solution has not been evaluated. However, other commercially available hemoglobin solutions, intended for use as microbiological medium supplements, are reportedly stable for more than 1 year when stored at 2-8°C.
  
  Helpful?
a year ago

Can it be reconstituted to a final concentration of 17 g/dL?

1 answer
1. Technical Support
  
  a year ago
  
  A concentration of 17 g/dL exceeds the solubility recommendation. Each batch released is tested and guaranteed to dissolve in deionized water at up to 20 mg/mL.
  
  Helpful?
a year ago

What is this product H7379 lyophilized in? When I reconstitute in water should I account for sucrose or other buffer components?

1 answer
1. Technical Support
  
  a year ago
  
  The protein was dialyzed against deionized water before the lyophilization step.
  
  Helpful?
a year ago

I noticed that the solubility of H7379 in H2O is only 20 mg/mL. We are looking for Hb solution with concentration from 10 to 20 g/dL. Could you kindly advise whether the power can have higher solubility in any other liquid?

1 answer
1. Technical Support
  
  a year ago
  
  This product is tested for solubility at 20 mg/mL during quality control testing in water. Testing in other liquids for higher solubility has not been performed.
  
  Helpful?
2 years ago

What is the standard of this reagent?

1 answer
1. Technical Support
  
  a year ago
  
  This product contains a dark red to red-brown human hemoglobin powder. The standards specified in the Certificate of Analysis include low water content (≤ 15%) and an iron level within 0.25–0.35%. Tests for hepatitis B surface antigen, hepatitis C virus, and human immunodeficiency virus are negative.
  
  Helpful?
2 years ago

This product lists HBG in the Gene list. Can you confirm that Hb gamma is present in this product?

1 answer
1. Technical Support
  
  a year ago
  
  The specification for this product does not require testing for Hb gamma. Information found under the Properties or Description sections of the Product Detail page is generally sourced from the literature. Below is the link to the Specification Sheet for reference: https://www.sigmaaldrich.com/specification-sheets/904/492/H7379-BULK________SIGMA____.pdf
  
  Helpful?
2 years ago

Hi, what is the bulk density of this product ?

1 answer
1. Technical Support
  
  2 years ago
  
  Unfortunately, the bulk density of this product is not determined.
  
  Helpful?

1–10 of 20 Questions

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Dieser Artikel	08449	3745	1303750
Sigma-Aldrich H7379 Hemoglobin human	Millipore 08449 Hemoglobin from bovine blood	Sigma-Aldrich 3745 Hemoglobin, Bovine Erythrocytes	USP 1303750 Hemoglobin Protease Substrate
technique(s) enzyme immunoassay: suitable	technique(s) -	technique(s) -	technique(s) -
biological source human	biological source bovine blood	biological source -	biological source -
form lyophilized powder	form powder	form lyophilized	form solid
impurities HIV and hepatitis B antigen, tested negative	impurities -	impurities -	impurities -
UniProt accession no. P02008, P02100, P68871, P69892, P09105, P69905, P02042, P69891	UniProt accession no. P01966, P02070, P02081, P06642, P06643	UniProt accession no. -	UniProt accession no. -
solubility H₂O: soluble 20 mg/mL	solubility -	solubility aqueous buffer: 1 mg/mL, water: 1 mg/mL	solubility -

SKU	GTIN
H7379-1G	04061833803721
H7379-5G	04061833803752
H7379-10G	04061835521876

Wichtige Dokumente

Fortfahren mit

Hämoglobin human

lyophilized powder

Größe auswählen

Über diesen Artikel

H7379-1G

€ 87,30

biological source

Quality Segment

form

technique(s)

impurities

solubility

UniProt accession no.

storage temp.

Gene Information

General description

Application

Biochem/physiol Actions

Disclaimer

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Dokumentation

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Questions

Tricia

Can I use this product to validate hemolysis testing on chemistry analyzer.

Bhavna

Would H7379 Hemoglobin Human has population of glycated hb as well? If yes, what level?

I would like to know if the product loses stability if it is kept in a freezer at -20, since the recommended temperature is between 2 and 8 degrees Celsius.

Anonymous

What are the recommendations for long-term storage once it is solubilized? Is it OK to make single-use aliquots for -80°C?

Pamela

Can it be reconstituted to a final concentration of 17 g/dL?

Shanon

What is this product H7379 lyophilized in? When I reconstitute in water should I account for sucrose or other buffer components?

IME, ASTAR

I noticed that the solubility of H7379 in H2O is only 20 mg/mL. We are looking for Hb solution with concentration from 10 to 20 g/dL. Could you kindly advise whether the power can have higher solubility in any other liquid?

Lisa

What is the standard of this reagent?

David

This product lists HBG in the Gene list. Can you confirm that Hb gamma is present in this product?

saeed najafian

Hi, what is the bulk density of this product ?

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