Yes, the difference between product H3667 and H4522, is that product H3667 is heat inactivated. Any availability questions can be directed to customer service. Please navigate to the link below:https://www.sigmaaldrich.com/support/customer-support.
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H3667
Humanserum
Heat Inactivated, from human male AB plasma, USA origin, sterile-filtered
Human Serum
Synonym(e):
Humanplasma, Humanseren, Humanserum
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| Packungsgröße | SKU | Verfügbarkeit | Preis |
|---|---|---|---|
| 20 mL | Warenkorb auf Verfügbarkeit prüfen | € 135,00 | |
| 100 mL | Warenkorb auf Verfügbarkeit prüfen | € 431,00 |
Über diesen Artikel
Biological source:
human male plasma (Type AB)
Origin:
USA origin
Sterility:
sterile-filtered
Shipped in:
dry ice
€ 135,00
Warenkorb auf Verfügbarkeit prüfen
Technischer Dienst
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Unterstützung erhaltenbiological source
human male plasma (Type AB)
Quality Segment
sterility
sterile-filtered
composition
hemoglobin, ≤20 mg/dL
origin
USA origin
technique(s)
cell culture | mammalian: suitable
impurities
≤10 EU/mL endotoxin
shipped in
dry ice
storage temp.
−20°C
General description
Humanserum wurde als Supplement in Nährmedien für Monozyten verwendet. Es enthält Makromoleküle, Trägerproteine, Bindungs- und Verbreitungsfaktoren, niedermolekulare Nährstoffe sowie Hormone und Wachstumsfaktoren.
Application
Humanserum wurde als Supplement in Nährmedien für Monozyten verwendet.[1] Es wurde als Kontrolle im Western Blot zur Untersuchung der Flightless-I-Expression in Serum eingesetzt.
Packaging
Die Verpackung kann sich aufgrund einer Knappheit der Flaschen, die zum Verpacken dieses Produkts verwendet werden, bei verschiedenen Chargen unterscheiden. Das Vorhandensein oder Fehlen von Schrumpffolie um den Flaschendeckel beeinträchtigt die Integrität/Stabilität des Produkts nicht.
Analysis Note
Endotoxin-getestet
Other Notes
Ein trübes Aussehen unserer Humanserumprodukte ist nicht ungewöhnlich. Dieses Produkt besteht aus gepooltem Serum. Eine Trübung zeigt an, dass das Serum Lipide enthält. Klare, lipidfreie Humanserumprodukte sind nicht erhältlich.
Disclaimer
NUR FÜR FORSCHUNGSZWECKE. Dieses Produkt unterliegt in Frankreich den Vorschriften für den Gebrauch zu wissenschaftlichen Zwecken, auch bei der Ein- und Ausfuhr (Artikel L 1211-1 Absatz 2 des französischen Gesundheitsgesetzes). Der Käufer (d. h. der Endverbraucher) ist verpflichtet, eine Einfuhrgenehmigung des französischen Forschungsministeriums gemäß Artikel L1245-5-1 II. des französischen Gesundheitsgesetzes einzuholen. Mit der Bestellung dieses Produkts bestätigen Sie, dass Sie über die entsprechende Einfuhrgenehmigung verfügen.
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Dieser Artikel | |||
|---|---|---|---|
| origin USA origin | origin USA origin | origin USA origin | origin USA origin |
| sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered | sterility sterile-filtered |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, cell culture | stem cell: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable, cell culture | stem cell: suitable |
| biological source human male plasma (Type AB) | biological source human male plasma (Type AB) | biological source human | biological source human |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| impurities ≤10 EU/mL endotoxin | impurities ≤10 EU/mL endotoxin | impurities (mycoplasma tested, virus tested), ≤10 EU/mL endotoxin | impurities ≤10 EU/mL Endotoxin |
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Lagerklasse
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
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Artikel
Find answers to frequently asked questions (FAQs) about FBS and other serum products, and links to related products.
A human and animal model-based approach to investigating the anti-inflammatory profile and potential of the 5-HT2B receptor antagonist AM1030.
Palmqvist N et al.
Journal of Inflammation (London, England), 13, 20-20 (2016)
Cytoskeletal protein Flightless I inhibits apoptosis, enhances tumor cell invasion and promotes cutaneous squamous cell carcinoma progression.
Kopecki Z et al.
Oncotarget, 6, 36426-36426 (2015)
Christina Megli et al.
The Journal of experimental medicine, 218(1) (2020-09-26)
The human placenta is a dynamic organ that modulates physiological adaptations to pregnancy. To define the immunological signature of the human placenta, we performed unbiased profiling of secreted immune factors from human chorionic villi isolated from placentas at mid and
Global Trade Item Number
| SKU | GTIN |
|---|---|
| H3667-100ML | 04061833793893 |
| H3667-20ML | 04061833793916 |
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Is the only difference between H3667 and H4522 that H3667 is heat inactivated whilst H4522 is not? And what makes a big difference in the availability between them?
1 answer-
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Is this tested for Anti HLA antibodies? I am thinking of it’s application as a flow cytometric negative control
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This product has not been tested for the presence of anti-HLA antibodies.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Why is serum Heat-Inactivated (HI)?
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Heat inactivation of serum is performed to inactivate complement (which can cause cell lysis or downstream problems in immunoassays). If there are immunoglobulins present in the serum that can bind to the cells in culture (either specifically or non-specifically), there is a chance for antibody-mediated complement cytotoxicity (ADCC, causing cell death). It may also reduce the titer of some mycoplasma and some viruses. Often, heat-inactivated serum is used because of the adoption of a previous protocol. It is mainly used when culturing hematopoetic cells or performing immunological type assays such as mixed-lymphocyte reactions or neutralization assays.FBS may not be heat inactivated as this is not required for all applications. It limits the loss of the other protein activity that may be minimally present in the serum.A protocol for heat inactivation:1. Allow serum containers to come to room temperature. Swirl bottles of serum immediately before adding to the water bath.2. A water-filled control bottles (T1) should be placed in a 56 °C water bath. Place the containers in the water bath up to the serum line. Do not completely submerge the containers. When the temperature of T1 reaches 56 °C, start the timer set for 30 minutes. Do not allow temperatures to exceed 56 °C. Higher temperatures will denature the proteins, gel the serum or alter the performance of the serum.3. Gently swirl the bottles every 5 to 10 minutes while in the water bath and check the temperature in the control bottle. It is not uncommon for plastic containers to bow slightly after heat treatment. This is not harmful to the serum.4. At the end of 30 minutes remove the serum bottles from the water bath. Aliquot the serum into smaller volumes and freeze. Sigma-Aldrich does not recommend repeated thaw-freeze cycles.
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What is the difference between human serum from AB plasma and human serum from AB clotted whole blood?
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Product H6914 is prepared from whole blood that is allowed to clot, centrifuged and the serum removed. There is no additional processing required.Product H4522 is prepared from anticoagulated (sodium citrate) whole blood. This is centrifuged to pellet the cells (RBC, WBC, some platelets) and the plasma is removed. This plasma is then treated with calcium to activate the clotting cascade. The preparation is centrifuged again and the supernatant is removed. This product can also be called plasma derived serum.Product H6914 will contain more growth factors since it is allowed to spontaneously clot and there will be the release of growth factors from many of the cells (WBC, platelets) present. The cells are removed before the plasma is clotted for Product H4522, so this plasma will have fewer growth factors present (more similar to what is circulating). Unless testing for growth factors, most researchers are looking for Product H6914.We offer both products heat inactivated.Product H5667 Human Serum Heat Inactivated, from male AB clotted whole bloodProduct H3667 Human Serum Heat Inactivated, from human male AB plasma
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