G9637

Glycerin-3-Phosphat-Oxidase aus Pediococcus sp.

lyophilized powder, 40-80 units/mg solid, pH 8.1

• Synonym(e): sn-Glycerol 3-phosphate:oxygen 2-oxidoreductase, L-Glycerin-3-phosphat-Oxidase
• CAS-Nummer: 9046-28-0
• UNSPSC Code: 12352204
• NACRES: NA.54
• EC Number: 232-932-0
• MDL number: MFCD00131209
• EG-Nummer: 1.1.3.21 (BRENDA, IUBMB)
• Specific activity: 40-80 units/mg solid, pH 8.1
• Biological source: bacterial (Pediococcus spp.)

Eigenschaften

• biological source: bacterial (Pediococcus spp.)
• Quality Segment: 200
• form: lyophilized powder
• specific activity: 40-80 units/mg solid, pH 8.1
• mol wt: ~76 kDa (gel filtration)
• storage temp.: 2-8°C

Beschreibung

General description

Glycerol 3-phosphate Oxidase is a FAD-dependent α-glycerophosphate oxidase and catalyzes the formation of dihydroxyacetonephosphate. Glycerol 3-phosphate oxidase comprises of a flavin adenine dinucleotide (FAD) and a substrate binding domain and corresponds to molecular weight of 42 kDa. It exists as a monomer and the active site comprises of isoalloxazine ring and histidine 53, arginine 316 and lysine 345 residues crucial for ligand binding.

Isoelectric point : 4.1 -/+0.1
Michaelis constants : 3.2x10 ^-3M (L-α-Glycerophosphate), 6.8 x 10 ^-3M (D, L-form)
Inhibitors : Ionic detergents (SDS, LBS, etc.), Hg⁺⁺, Ag⁺
Optimum pH : 8.0 - 8.5Optimum temperature : 35 - 40o^C
pH Stability : pH 6.5 - 8.5 (25o^C, 20hr) Thermal stability : below 40oC (pH 7.0, 15min)
Substrate specificity : The enzyme has the highest specificity for L-form of a-γlycerophosphate

Application

Glycerol 3-phosphate Oxidase from Pediococcus sp. has been used in the kinetic assay to study gluconeogenesis in liver and in immobilization studies on pencil graphite (PG) electrode.

This enzyme is useful for enzymatic determination of triglyceride when coupled with lipoprotein lipase (LPL-311, LPL-314) and glycerokinase (GYK-301, GYK-311) in clinical analysis.

Physical form

Lyophilized powder containing stabilizers

Other Notes

One unit will oxidize 1.0 μmole of L-glycerol 3-phosphate to dihydroxyacetone phosphate with the formation of H₂O₂ per min at 37°C, at the appropriate pH.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)