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G8644

D-(+)-Glukose -Lösung

100 g/L in H2O, sterile-filtered, BioXtra, suitable for cell culture

Synonym(e):

D-Glucopyranose

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Über diesen Artikel

Empirische Formel (Hill-System):
C6H12O6
CAS-Nummer:
50-99-7
Molekulargewicht:
180.16
MDL number:
MFCD00063774
UNSPSC Code:
12352201
PubChem Substance ID:
24895346
NACRES:
NA.75
Beilstein/REAXYS Number:
1281608
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
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Unterstützung erhalten

sterility

sterile-filtered

Quality Level

400

product line

BioXtra

form

solution

concentration

100 g/L in H2O

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

storage temp.

room temp

SMILES string

OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@@H]1O

InChI

1S/C6H12O6/c7-1-2-3(8)4(9)5(10)6(11)12-2/h2-11H,1H2/t2-,3-,4+,5-,6+/m1/s1

InChI key

WQZGKKKJIJFFOK-DVKNGEFBSA-N

Gene Information

human ... PYGM(5837)

General description

Glucose ist die primäre Energiequelle für den Zellstoffwechsel. In lebenden Organismen ist sie die Hauptenergiequelle. Glucose kommt in ihrem freien Zustand von Natur aus in Früchten und anderen Pflanzenteilen vor. Sie wird zu Glucosiden, Disacchariden, Oligosacchariden, Polysacchariden (Zellulose und Stärke) sowie Glycogen kombiniert.

Application

D-(+)-Glucoselösung wird wie folgt verwendet:

  • zum Bestimmen der extrazellulären Versauerungsrate (ECAR) oder Sauerstoffverbrauchsrate (OCR) in humanen CB CD34+-Zellen mittels Seahorse-Assay des extrazellulären Flusses[1]
  • als Negativkontrolle in einer Kultur von humanen Nabelschnurendothelzellen (HUVEC)[2]
  • als Zusatz beim Kultivieren von Dickdarmbiopsien[3]

Biochem/physiol Actions

Glucose ist ein Monosaccharid (Aldose) und dient als wichtige Komponente der Glykolyse und nachgelagerten Wege für die aerobe und anaerobe Atmung. Sie liefert Energie für Wachstum und Reproduktion. Glucose ist ein Nebenprodukt der Photosynthese und wird in Pflanzen und Cyanobakterien zu Stärke kondensiert.[4]

Other Notes

Dieses Produkt wurde mit Zelllinien getestet, um eine Zytotoxizität des Produkts auszuschließen. Die wachstumsfördernde Fähigkeit dieser zellkulturgetesteten Glucoselösung wurde mit geeigneten Zelllinien in einem Medium von 45 mL/L beurteilt.
Diese Lösung wurde durch Filtration sterilisiert.

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Dieser Artikel
G8769G7021G7528
D-(+)-Glucose solution 100 g/L in H2O, sterile-filtered, BioXtra, suitable for cell culture

Sigma-Aldrich

G8644

D-(+)-Glucose solution

D-(+)-Glucose solution 45% in H2O, sterile-filtered, BioXtra, suitable for cell culture

Sigma-Aldrich

G8769

D-(+)-Glucose solution

D-(+)-Glucose powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.5%

Sigma-Aldrich

G7021

D-(+)-Glucose

D-(+)-Glucose ≥99.5% (GC), BioXtra

Sigma-Aldrich

G7528

D-(+)-Glucose

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | insect: suitable, cell culture | mammalian: suitable, cell culture | plant: suitable

technique(s)

gas chromatography (GC): suitable

Quality Level

400

Quality Level

200

Quality Level

200

Quality Level

200

form

solution

form

solution

form

powder

form

powder

storage temp.

room temp

storage temp.

-

storage temp.

room temp

storage temp.

room temp

product line

BioXtra

product line

BioXtra

product line

BioReagent

product line

BioXtra

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

Insoluble matter, passes filter test

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Lagerklasse

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
RNBL7494
RNBL1792
RNBK9929
RNBK3918
RNBJ8362

Die passende Version wird nicht angezeigt?

Wenn Sie eine bestimmte Version benötigen, können Sie anhand der Lot- oder Chargennummer nach einem spezifischen Zertifikat suchen.

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Verwandter Inhalt

Shu J Lam et al.
ACS applied materials & interfaces, 8(49), 33446-33456 (2016-12-15)
'Structurally nanoengineered antimicrobial peptide polymers' (SNAPPs), in the form of star-shaped peptide polymer nanoparticles, have been recently demonstrated as a new class of antimicrobial agents with superior in vitro and in vivo efficacy against Gram-negative pathogens, including multidrug-resistant species. Herein
Brandon Mapes et al.
Physiological genomics, 46(8), 302-308 (2014-02-20)
1α,25-Dihydroxyvitamin D3 [1α,25(OH)2D3] is a steroid hormone derived from circulating 25(OH) vitamin D [25(OH)D] with chemopreventive effects in colorectal cancer. 1α,25(OH)2D3 acts through transcriptional mechanisms; however, our understanding of vitamin D transcriptional responses in the colon is derived from studies
Qianqian Li et al.
Journal of cellular and molecular medicine, 24(1), 910-920 (2019-11-05)
In type 1 and type 2 diabetes mellitus, increased cardiac fibrosis, stiffness and associated diastolic dysfunction may be the earliest pathological phenomena in diabetic cardiomyopathy. Endothelial-mesenchymal transition (EndMT) in endothelia cells (ECs) is a critical cellular phenomenon that increases cardiac
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Global Trade Item Number

SKUGTIN
G8644-10L04061837035272
G8644-100ML04061833649718

Questions

1–5 of 5 Questions  

  1. Hello what is the recommended concentrations of D-glucose solution 100g/l to used for primary cell culture of brain ?

    1 answer

    1. There are a number of cell types present in brain tissue. Glucose requirements may vary by cell type. This product is not tested for use in primary cell culture. However, the typical glucose concentration in most classical media is 1 g/L or 5 g/L. In brain slice or hippocampal slice studies, the glucose concentration may vary broadly. It is advisable to search to the literature for the appropriate concentration of D-glucose to use for primary brain cell culture. Please see the links below to review examples of these publications:

      'Organotypic hippocampal slice culture from the adult mouse brain: A versatile tool for translational neuropsychopharmacology'
      https://www.sciencedirect.com/science/article/pii/S0278584612002862?via%3Dihub

      'Preparation of organotypic brain slice cultures for the study of Alzheimer’s disease'
      https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5964634/

      Helpful?

  2. What is the Molar concentration of Product G8769, D-(+)-Glucose solution?

    1 answer

    1. The molar concentration of Glucose solutions G8644 and G8769, may only be estimated. The glucose molecular weight is 180.16 g/mol, however the concentration is lot specific.

      Product G8644 has a concentration specification ranging from 90 - 110 g/L or 0.50 - 0.61 M.

      Please see the link below to access a sample or lot-specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/sigma/g8644#product-documentation

      Product G8769 has a concentration specification ranging from 405 - 495 g/L or 2.24 - 2.74 M.

      Please see the link below to access a sample or lot-specific Certificate of Analysis:
      https://www.sigmaaldrich.com/product/sigma/g8769#product-documentation

      Helpful?

  3. How does the storage temperature relate to shipping conditions?

    1 answer

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      Helpful?

  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  5. What is the Molar concentration of Product G8644, D-(+)-Glucose solution?

    1 answer

    1. Based on a 10% (w/v) concentration and a molecular weight for glucose of 180 Da,  Product G8644 D-(+)-Glucose solution, is 0.56 M glucose.

      Helpful?

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