F8435

PNGase F Glycosidase

Removes N-linked glycans, suitable for mass spectrometry, recombinant, expressed in E. coli

• Synonym(e): N-Glycosidase F, PNGase F aus Chryseobacterium meningosepticum, PNGase F aus Flavobacterium meningosepticum, Peptid-N-Glycosidase
• CAS-Nummer: 83534-39-8
• UNSPSC Code: 12352204
• NACRES: NA.54
• EG-Nummer: 3.5.1.52 (BRENDA, IUBMB)
• MDL number: MFCD18839214
• Specific activity: ≥1,000 U/mg
• Recombinant: expressed in E. coli
• Shelf life: ≥1 weeks at 2‑8 °C (for reconstituted solution), ≥1 yr at -20 °C

Eigenschaften

• Produktname: PNGase F aus Elizabethkingia meningoseptica, lyophilized powder, recombinant, expressed in E. coli
• recombinant: expressed in E. coli
• Quality Segment: 200
• conjugate: (N-linked)
• grade: Proteomics Grade
• form: lyophilized powder
• specific activity: ≥1,000 U/mg
• shelf life: ≥1 weeks at 2‑8 °C (for reconstituted solution), ≥1 yr at -20 °C
• mol wt: ~36 kDa
• shipped in: wet ice
• storage temp.: −20°C

Beschreibung

Application

Wird zur Deglykolisierung von Protein verwendet.

Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.

Biochem/physiol Actions

Spaltet ein gesamtes Glykan von einem Glykoprotein ab, vorausgesetzt, die glykolysierte Asparagineinheit ist an ihrem Amino- und Carboxyterminus mit einer Polypeptidkette substituiert.

Packaging

PNGase F was used for deglycosylation of P-glycoprotein in a study to investigate the dual impact of statins on p-glycoprotein and its effect on doxorubicin cytotoxicity in human neuroblastoma cells.[1] It was used to treat a purified protein, mouse cone ultraviolet (MUV) pigment, before use in quantitative immunoblot analysis in a study[2] It is used to deglycosylate N-linked glycoproteins. Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.

Other Notes

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Sicherheitshinweise

• pictograms: GHS08
• signalword: Danger
• hcodes: H334
• pcodes: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable