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F8435

Sigma-Aldrich

PNGase F aus Elizabethkingia meningoseptica

lyophilized powder, recombinant, expressed in E. coli

Synonym(e):

N-Glycosidase F, PNGase F aus Chryseobacterium meningosepticum, PNGase F aus Flavobacterium meningosepticum, Peptid-N-Glycosidase

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€ 220,00
300 UNITS
€ 1.040,00

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50 UNITS
€ 220,00
300 UNITS
€ 1.040,00

About This Item

CAS-Nummer:
83534-39-8
EC-Nummer:
3.5.1.52 (BRENDA, IUBMB)
MDL-Nummer:
MFCD00131222
UNSPSC-Code:
12352204
NACRES:
NA.54

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Rekombinant

expressed in E. coli

Qualitätsniveau

200

Konjugat

(N-linked)

Qualität

Proteomics Grade

Form

lyophilized powder

Spezifische Aktivität

≥1,000 U/mg

Haltbarkeit

≥1 weeks at 2‑8 °C (for reconstituted solution)
≥1 yr at -20 °C

Mol-Gew.

~36 kDa

Versandbedingung

wet ice

Lagertemp.

−20°C

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Verwandte Kategorien

Anwendung

Wird zur Deglykolisierung von Protein verwendet.
Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.

Biochem./physiol. Wirkung

Spaltet ein gesamtes Glykan von einem Glykoprotein ab, vorausgesetzt, die glykolysierte Asparagineinheit ist an ihrem Amino- und Carboxyterminus mit einer Polypeptidkette substituiert.

Verpackung

PNGase F was used for deglycosylation of P-glycoprotein in a study to investigate the dual impact of statins on p-glycoprotein and its effect on doxorubicin cytotoxicity in human neuroblastoma cells.[1] It was used to treat a purified protein, mouse cone ultraviolet (MUV) pigment, before use in quantitative immunoblot analysis in a study[2] It is used to deglycosylate N-linked glycoproteins. Highly purified material can be used for preparative deglycosylation or for analytical applications in gel, in solution, or on blot membranes. The enzyme can be removed from preparative operations by utilizing its C-teminal 6x histidine fusion tag.

Einheitendefinition

One unit will catalyze the release of N-linked oligosaccharides from 1 nanomole of denatured ribonuclease B in one minute at 37°C at pH 7.5 monitored by SDS-PAGE. One Sigma unit of PNGase F activity is equal to 1 IUB milliunit.

Piktogramme

Health hazard
GHS08

Signalwort

Danger

H-Sätze

H334

Gefahreneinstufungen

Resp. Sens. 1

Lagerklassenschlüssel

11 - Combustible Solids

WGK

WGK 3

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number
0000432197
0000432197
0000405902
0000405901
0000405901

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Maria Nordgren et al.
Methods in molecular biology (Clifton, N.J.), 2271, 155-167 (2021-04-29)
O-glycosylation is a difficult posttranslational modification to analyze. O-glycans are labile and often cluster making their analysis by LC-MS very challenging. OpeRATOR is an O-glycan specific protease that cleaves the protein backbone N-terminally of glycosylated serine and threonine residues. This
T H Plummer et al.
The Journal of biological chemistry, 259(17), 10700-10704 (1984-09-10)
Endo-beta-N-acetylglucosaminidase F preparations from Flavobacterium meningosepticum have been found to contain peptide:N-glycosidase activity. Only the second activity, designated as peptide:N-glycosidase F, readily cleaves the beta-aspartylglycosylamine linkage of a fetuin triantennary complex glycopeptide, as shown by the isolation of the corresponding
Evelyn Sieczkowski et al.
International journal of cancer, 126(9), 2025-2035 (2009-09-10)
The development of multidrug resistance (MDR) is a major problem during cancer treatment. Drug efflux via ATP-binding cassette (ABC) transporters is the main mechanism responsible for resistance to chemotherapeutics. We have recently observed that statins enhance susceptibility to doxorubicin-induced apoptosis
Tobias Bierig et al.
Frontiers in bioengineering and biotechnology, 8, 618615-618615 (2021-01-08)
2019-nCoV is the causative agent of the serious, still ongoing, worldwide coronavirus disease (COVID-19) pandemic. High quality recombinant virus proteins are required for research related to the development of vaccines and improved assays, and to the general understanding of virus
Lauren L Daniele et al.
Investigative ophthalmology & visual science, 46(6), 2156-2167 (2005-05-26)
To test the hypothesis that Nrl(-)(/)(-) photoreceptors are cones, by comparing them with WT rods and cones using morphological, molecular, histochemical, and electrophysiological criteria. The photoreceptor layer of fixed retinal tissue of 4- to 6-week-old mice was examined in plastic
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