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F7425

ANTI-FLAG^® in Kaninchen hergestellte Antikörper

Name: ANTI-FLAG<SUP>®</SUP> in Kaninchen hergestellte Antikörper
Brand: SIGMA
Price: 605 EUR

affinity isolated antibody, buffered aqueous solution

ANTI-FLAG® antibody produced in rabbit

Synonym(e):

Anti-ddddk, Anti-dykddddk

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Packungsgröße	SKU	Verfügbarkeit	Preis
0.2 mg		Warenkorb auf Verfügbarkeit prüfen	€ 605,00

Über diesen Artikel

UNSPSC Code:

12352203

NACRES:

NA.32

MDL number:

MFCD02262912

Conjugate:

unconjugated

Clone:

polyclonal

Application:

DB, IF, IP, WB CL

Citations:

3023

€ 605,00

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Technischer Dienst

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Unterstützung erhalten

Eigenschaften

biological source

rabbit

Quality Segment

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

all

concentration

~0.8 mg/mL

technique(s)

dot blot: 1-2.5 μg/mL, immunoprecipitation (IP): 4-8 μg using amino terminal FLAG-BAP fusion protein from E. coli crude lysate, indirect immunofluorescence: 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK, western blot (chemiluminescent): 1-2.5 μg/mL using an E. coli periplasmic extract expressing an N-terminal FLAG fusion protein

isotype

IgG

immunogen sequence

DYKDDDDK

shipped in

dry ice

storage temp.

−20°C

Beschreibung

General description

Der polyklonale Anti-FLAG-Kaninchenantikörper ANTI-FLAG erkennt das FLAG-Epitop auf FLAG-Fusionsproteinen. Dieser Antikörper reagiert mit N-terminalen, Met-N-terminalen und C-terminalen FLAG-Fusionsproteinen.

Das FLAG-Epitop ist ein Protein aus acht Aminosäuren mit einer Enterokinase-Spaltstelle.

Immunogen

FLAG; Peptid-Sequenz DYKDDDDK

Application

Der Antikörper erkennt das FLAG-Epitop auf FLAG-markierten Fusionsproteinen am N-Terminus oder C-Terminus in Dot-Blot-, Immunblot-, Immunpräzipitations- und Immunzytochemie-Assays.
Weitere Produktinformationen finden Sie in unserem FLAG® Literatur-Portal.

Biochem/physiol Actions

Das FLAG-Epitop spielt eine entscheidende Rolle bei der Immunaffinitätsaufreinigung von Fusionsproteinen.

Physical form

Lösung in 0,01 M phosphatgepufferter Kochsalzlösung, pH 7,4, enthält 1 % Rinderserumalbumin und 15 mM Natriumazid

Preparation Note

Mittels Affinitätschromatographie auf einer Säule mit dem immunisierenden Peptid aufgereinigt.

Other Notes

Gewinner des CiteAb Award 2024 in der Kategorie „Supplier Succeeding in Parkinson′s Research“ (Erfolgreiche Anbieter in der Parkinson-Forschung)

Legal Information

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

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Dieser Artikel	F2555	F3165	SAB4200119
Millipore F7425 ANTI-FLAG^® antibody produced in rabbit	Sigma-Aldrich F2555 Monoclonal ANTI-FLAG^® antibody produced in rabbit	Sigma-Aldrich F3165 ANTI-FLAG^® M2 antibody, Mouse monoclonal	Sigma-Aldrich SAB4200119 Monoclonal Anti-FLAG-Peroxidase antibody produced in rat
species reactivity all	species reactivity -	species reactivity all	species reactivity all
clone polyclonal	clone SIG1-25, monoclonal	clone M2, monoclonal	clone 6F7, monoclonal
conjugate unconjugated	conjugate unconjugated	conjugate unconjugated	conjugate peroxidase conjugate
biological source rabbit	biological source rabbit	biological source mouse	biological source rat
antibody form affinity isolated antibody	antibody form ascites fluid	antibody form purified immunoglobulin (Purified IgG1 subclass)	antibody form purified immunoglobulin
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice

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Lagerklasse

12 - Non Combustible Liquids

flash_point_f

Not applicable

flash_point_c

Not applicable

Hier finden Sie alle aktuellen Versionen:

Analysenzertifikate (COA)

Lot/Batch Number

0000485141

0000422515

0000395474

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Global Trade Item Number

SKU	GTIN
F7425-.2MG	04061838255501

Questions

1–9 of 9 Questions

3 years ago

Can this AB (F7425) used for mouse 30um thick section. mouse will be perfused with 4% PFA and than cryopreserved. thanks

1 answer
1. Technical Support
  
  3 years ago
  
  This product has been recommended for use in immunofluorescence and indirect immunofluorescence at 5-10 μg/mL using 293T cells transfected with a plasmid encoding FLAG-JNK. It has not been tested for use in IHC applications. The end-user will need to optimize the assay. Please see the link below to review the product datasheet:
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/207/353/f7425dat-ms.pdf
  In general, antibodies work well on 5-15 uM sections, for 30 uM sections, blocking /permeabilization steps and antibody dilution, the incubation time will need to be optimized by end user. Please see the links below to review an alternative anti-Flag antibody that may be more suitable for this application. Note that this product is a mouse monoclonal antibody:
  https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/175/747/f1804bul-ms.pdf
  https://www.sigmaaldrich.com/product/sigma/f1804
  
  Helpful?
3 years ago

Is there a recommended isotype control for this antibody?

1 answer
1. Technical Support
  
  3 years ago
  
  Any rabbit IgG such as product I5006 would be an appropriate isotype control for this antibody.
  
  Helpful?
Anonymous

4 years ago

Is there any references for the use of Product F7465, ANTI-FLAG® from rabbit, in immunohistochemistry or western blotting?

1 answer
1. Technical Support
  
  4 years ago
  
  K. Schafer and T. Braun, Monoclonal Anti-FLAG Antibodies React with a New Isoform of Rat Mg++ Dependent Protein Phosphatase. Biochemical and Biophysical Research Communications, 207, 708.
  
  Helpful?
Anonymous

4 years ago

Is Product F7425, ANTI-FLAG® from rabbit, a monoclonal or polyclonal?

1 answer
1. Technical Support
  
  4 years ago
  
  F7425 is a polyclonal antibody from rabbit.
  
  Helpful?
Anonymous

4 years ago

What is the Department of Transportation shipping information for this product?

1 answer
1. Technical Support
  
  4 years ago
  
  Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
  
  Helpful?
Anonymous

4 years ago

What is the concentration of Product F7425, ANTI-FLAG® from rabbit?

1 answer
1. Technical Support
  
  4 years ago
  
  The concentration is 0.9 mg protein per ml.
  
  Helpful?
Anonymous

4 years ago

What are the positive controls that can be used with Product F7425, ANTI-FLAG® from rabbit?

1 answer
1. Technical Support
  
  4 years ago
  
  The positive controls that can be used are as follows:Amino-terminal FLAG-BAP fusion protein, Product No. P7582Carboxy-terminal FLAG-BAP fusion protein, Product No. P7457Amino-terminal Met-FLAG-BAP fusion protein, Product No. P5975.
  
  Helpful?
Anonymous

4 years ago

How to get rid of background when using Product F7465, ANTI-FLAG® from rabbit,?

1 answer
1. Technical Support
  
  4 years ago
  
  To reduce non-specific background on tissue staining, a small amount of Triton X-100 (Product No. X100 or T8787), can be added to the wash buffers and antibody incubation buffers. Try a Triton X-100 concentration of 0.1% to start. Tween 20 (Product No. P5927) can also be used.
  
  Helpful?
Anonymous

4 years ago

How much Product F7425, ANTI-FLAG® from rabbit, should be used in western blotting and ELISA applications?

1 answer
1. Technical Support
  
  4 years ago
  
  For a Western blot from a PAGE run (preferably) under denaturing conditions, a 1:400 dilution of this antibody should be used. A 1-10 μg/ml concentration should be used when using thie antibody in an ELISA application.
  
  Helpful?

Reviews

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Ash Bakhtiari
Ash Bakhtiari

Nottingham
Review 1

Votes 0
5 out of 5 stars.

Ash Bakhtiari

2 years ago

Western blotting
I HAVE USED THIS ANTIBODY ALOT DURING MY RESEARCH. AND IT HAS WORKED PERFECTLY.

A donation to charity was made for this review

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